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目的:研究氯化钴(CoCl2)对大鼠胚胎心脏来源的H9c2心肌细胞中新基因Mipu1表达的影响。方法:利用不同浓度的CoCl2(0、100、200、300、400、500μmol/L)处理H9c2细胞9h,及200μmol/L CoCl2处理H9c2细胞不同的时间(0、6、9、12、24h)后,用RT-PCR和Western Blot分别观察H9c2细胞Mipu1 mRNA和蛋白的表达情况。结果:CoCl2可以诱导H9c2细胞中Mipu1 mRNA和蛋白表达升高,200μM CoCl2处理组的Mipu1的表达水平高于100μM CoCl2处理组,但是更高浓度的CoCl2(>200μM)不能使Mipu1的表达进一步升高。随着CoCl2作用时间的延长,Mipu1的表达逐步升高,在12h达到高峰,但是在24h后下降。结论:CoCl2能够促进H9c2细胞新基因Mipu1的表达,并且具有一定的剂量和时间依赖性。
AIM: To investigate the effect of cobalt chloride (CoCl2) on the expression of Mipu1, a novel gene in rat embryonic cardiac H9c2 cardiomyocytes. Methods: H9c2 cells were treated with different concentrations of CoCl2 (0,100,200,300,400,500μmol / L) for 9h and H9c2 cells treated with 200μmol / L CoCl2 for different time (0,6,9,12,24h) The expression of Mipu1 mRNA and protein in H9c2 cells was detected by RT-PCR and Western Blot respectively. Results: CoCl2 induced the expression of Mipu1 mRNA and protein in H9c2 cells. Mipu1 expression in 200μM CoCl2 treatment group was higher than that in 100μM CoCl2 treatment group, but higher concentrations of CoCl2 (> 200μM) could not increase Mipu1 expression . With the extension of the action time of CoCl2, the expression of Mipu1 gradually increased and peaked at 12h, but decreased after 24h. Conclusion: CoCl2 can promote the expression of Mipu1, a novel gene in H9c2 cells, with a dose and time dependent manner.