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目的以牛角瓜花为实验材料,对其抑菌活性进行研究。方法以索氏提取法得到牛角瓜花的石油醚、氯仿、乙酸乙酯和甲醇的粗提物,并选择白色念珠菌等6种真菌和金黄色葡萄球菌等7种细菌,采用纸片琼脂扩散法对不同溶剂粗提物进行抑菌活性测定。结果牛角瓜花的石油醚粗提物对黄曲霉、枯草芽孢杆菌和尖孢镰刀菌表现出优秀的抑菌效果,抑菌圈直径分别为13.37、12.81、12.25 mm,均优于阳性对照氟康唑和两性霉素B的抑菌活性;氯仿粗提物对枯草芽孢杆菌、大肠埃希菌和白色念珠菌表现出良好的抑菌活性,抑菌圈直径分别为11.73、11.10、10.84 mm;乙酸乙酯和甲醇粗提物的抑菌效果较弱,乙酸乙酯粗提物对黄曲霉的抑菌圈直径为12.81 mm,甲醇粗提物对黑曲霉的抑菌圈直径为9.90 mm,且两种粗提物对其他供试菌有较弱或无抑菌效果。结论牛角瓜花中的化学成分在不同溶剂中的溶解性不同,使得4种溶剂粗提物的抑菌能力存在差异。
Objective To study the antibacterial activity of horn melon flower. Methods Crude extract of petroleum ether, chloroform, ethyl acetate and methanol was obtained by Soxhlet extraction. Six kinds of bacteria including Candida albicans and Staphylococcus aureus were selected, Act on different solvents crude extract antibacterial activity determination. Results Crude petroleum ether crude extract showed good antibacterial activity against Aspergillus flavus, Bacillus subtilis and Fusarium oxysporum, and the diameter of inhibition zone was 13.37, 12.81 and 12.25 mm, respectively, which were better than that of the positive control Fluconazole Azole and amphotericin B. The crude extract of chloroform showed good antibacterial activity against Bacillus subtilis, Escherichia coli and Candida albicans, and the diameters of inhibition zones were 11.73, 11.10 and 10.84 mm, respectively. Acetic acid The antibacterial effect of crude extracts of ethyl acetate and methanol was weak, the inhibition zone diameter of crude extract of ethyl acetate to Aspergillus flavus was 12.81 mm, the diameter of inhibition zone of crude extract of methanol to Aspergillus niger was 9.90 mm, Crude extract of other test bacteria have weaker or no antibacterial effect. Conclusion The chemical constituents in the melon flower have different solubility in different solvents, which makes the antibacterial ability of crude extract of four solvents different.