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目的探讨重度子痫前期患者胎盘Axl的表达及与其发病的关系。方法选择2012年4月至2012年10月在四川大学华西第二医院就诊的孕产妇。分别采用免疫组织化学法、蛋白印迹、酶联免疫吸附及实时荧光定量RT-PCR法检测胎盘Axl蛋白及mRNA的表达。采用方差分析、t检验等方法比较各组间胎盘Axl蛋白及mRNA的表达强度,采用Pearson或Spearman’s法进行相关性分析。结果 (1)重度子痫前期患者及正常妊娠者胎盘母面及子面同时存在Axl蛋白的表达部位一致,均定位于胎盘的血管内皮细胞。(2)重度子痫前期患者及正常妊娠者各自胎盘母面及子面Axl蛋白的表达无统计学差异。(3)重度子痫前期患者胎盘Axl蛋白的表达水平显著高于正常妊娠组,差异有统计学意义(P<0.05)。(4)重度子痫前期患者胎盘Axl-mRNA表达水平显著低于正常妊娠者,差异有统计学意义(P<0.05)。(5)重度子痫前期患者胎盘Axl蛋白表达水平与分娩前收缩压、舒张压及24 h尿蛋白呈正相关,与分娩孕周呈负相关;正常妊娠者胎盘Axl蛋白与各临床指标无明显相关性。结论重度子痫前期患者存在胎盘Axl蛋白及mRNA表达的异常;其胎盘Axl蛋白表达水平与疾病的某些重要临床指标相关。上述研究提示Axl的异常表达可能参与子痫前期的发病机制。
Objective To investigate the relationship between the expression of Axl in placenta and its pathogenesis in severe preeclampsia. Methods From April 2012 to October 2012, we visited the second West China Hospital of Sichuan University. The expression of Axl protein and mRNA in placenta were detected by immunohistochemistry, Western blotting, enzyme - linked immunosorbent assay and real - time fluorescent quantitative RT - PCR. The analysis of variance and t test were used to compare the expression of Axl protein and mRNA in the placenta between groups. Pearson or Spearman’s method was used to analyze the correlation. Results (1) The expression of Axl protein was consistent in both mother and child placenta of patients with severe preeclampsia and normal pregnancy, all located in the placental vascular endothelial cells. (2) There was no significant difference in the expression of Axl protein between the placenta and placenta of patients with severe preeclampsia and normal pregnancy. (3) The expression of Axl protein in placenta of patients with severe preeclampsia was significantly higher than that of normal pregnancy group (P <0.05). (4) The expression of Axl-mRNA in placenta of patients with severe preeclampsia was significantly lower than that of normal pregnancy (P <0.05). (5) The expression of Axl protein in placenta of patients with severe preeclampsia was positively correlated with systolic blood pressure, diastolic blood pressure and 24-hour urinary protein in preeclampsia, and was negatively correlated with gestational age. There was no significant correlation between Axl protein and placenta Sex. Conclusion There is abnormal expression of Axl protein and mRNA in placenta of patients with severe preeclampsia. The expression of Axl protein in placenta is related to some important clinical indicators of disease. These studies suggest that abnormal Axl expression may be involved in the pathogenesis of preeclampsia.