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目的:研究整合素αvβ6小干扰RNA对人卵巢癌HO-8910PM细胞侵袭生长的抑制作用。方法:以脂质体法将整合素αvβ6-siRNA(siRNA组)及无义寡核苷酸质粒(Neo组)转染人卵巢癌HO-8910PM细胞,通过实时定量PCR及蛋白质印迹试验,分别测定siRNA组、Neo组和空白对照组αvβ6整合素mRNA和蛋白的表达,MTT法和Transwell小室法分别测定其体外增殖能力和侵袭力。将各组细胞接种于裸小鼠皮下,观察移植瘤生长速度,并用免疫组化法检测整合素αvβ6的表达。结果:与Neo组和空白对照组相比较,siRNA组中αvβ6整合素mRNA和蛋白表达强度明显降低;转染的卵巢癌HO-8910PM细胞侵袭力明显下降(P<0.01);各组细胞体外增殖能力差异无统计学意义(P>0.05),但siRNA组细胞裸鼠皮下移植瘤生长速度明显低于其他两组,免疫组化显示,转染整合素αvβ6-siRNA的移植瘤中整合素αvβ6为阴性表达。结论:αvβ6整合素siRNA转染HO-8910PM细胞后,通过降低αvβ6整合素mRNA和蛋白表达水平,抑制其对细胞外基质的侵袭,进而抑制裸鼠移植瘤的生长。
Objective: To study the inhibitory effect of integrin αvβ6 small interfering RNA on the invasion and growth of human ovarian cancer HO-8910PM cells. Methods: Integrin αvβ6-siRNA (siRNA group) and non-sense oligonucleotide plasmid (Neo group) were transfected into human ovarian cancer HO-8910PM cells by lipofectamine. The expression of integrin αvβ6 siRNA was detected by real-time quantitative PCR and Western blotting siRNA group, Neo group and blank control group αvβ6 integrin mRNA and protein expression, MTT assay and Transwell chamber method were measured in vitro proliferation and invasiveness. The cells in each group were inoculated subcutaneously in nude mice to observe the growth rate of the xenografts and the expression of integrin αvβ6 was detected by immunohistochemistry. Results: Compared with Neo group and blank control group, the expression of αvβ6 integrin mRNA and protein in siRNA group was significantly decreased. The invasiveness of HO-8910PM cells in transfected ovarian cancer cells was significantly decreased (P <0.01). The proliferation of cells in vitro (P> 0.05). However, the growth rate of subcutaneous xenografts in nude mice in siRNA group was significantly lower than that in the other two groups. Immunohistochemistry showed that integrin αvβ6 in transplanted integrin αvβ6-siRNA was Negative expression. CONCLUSION: Transfection of αvβ6 integrin siRNA into HO-8910PM cells can inhibit the growth of xenografts in nude mice by decreasing the expression of αvβ6 integrin mRNA and protein and inhibiting the invasion of extracellular matrix.