Synthesis,Crystal Structure and Anti-tumor Activity of 2-(2-Chloro-4-nitrophenyl)-4-(4-chlorophenyl)

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The title compound 2-(2-chloro-4-nitrophenyl)-4-(4-chlorophenyl)-3a,4- diethoxy- 2,3,3a=4-tetrahydrochromeno[3,4-d][1,2,3]diazaphosphole 2 (C29H30Cl2N3O7P=Mr=633.44) was synthesized and its structure was characterized by IR=MS=1H NMR=13C NMR=31P NMR=elemental analysis and single-crystal X-ray diffraction. It crystallizes in triclinic=space group P1=a=9.1549(3)=b=10.7168(4)=c=17.6272(6)=α=102.9363(12)=β=90.2713(9)=γ=117.4265(10)°=V=1484.41(9)3=Z=2=μ(MoKα)=0.323=F(000)=658=Z=2=Dc=1.417 g/cm3=the final R=0.0687 and wR=0.2066 for 4943 observed reflections (I >2σ(I)). X-ray analysis reveals that the diazaphospholine ring is almost planar and the two ethoxy groups bonded on the 3a- and 4-positions are in trans configurations. Its antiproliferative activity was also tested in vitro against four human tumor cell lines. The title compound 2- (2-chloro-4-nitrophenyl) -4- (4- chlorophenyl) -3a, 4- diethoxy- 2,3,3a = 4-tetrahydrochromeno [3,4-d] 3] diazaphosphole 2 (C29H30Cl2N3O7P = Mr = 633.44) was synthesized and its structure was characterized by IR = MS = 1H NMR = 13C NMR = 31P NMR = elemental analysis and single-crystal X- ray diffraction. It crystallizes in triclinic = space group P1 = a = 9.1549 3 = b = 10.7168 4 = c = 17.6272  = α = 102.9363 = β = 90.2713 (9) = γ = 117.4265 (10) ° = V = 1484.41 9 = final 3 = Z = 2 = μ (MoKα) = 0.323 = F (000) = 658 = Z = 2 = Dc = 1.417 g / cm3 = the final R = 0.0687 and wR = 0.2066 for 4943 observed reflections (I> 2σ (I)). X-ray analysis reveals that the diazaphospholine ring is almost planar and the two ethoxy groups bonded on the 3a- and 4-positions are in trans configurations. Its antiproliferative activity was also tested in vitro against four human tumor cells lines.
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