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目的了解天津市腹泻患者副溶血性弧菌(vibrio parahaemolyticus,VP)感染状况、症状特征和病原学特征。方法在天津市7家哨点医院收集腹泻患者粪便样本和症状信息,并于24 h内检验分离其中的副溶血性弧菌,对阳性分离株进行血清学分型、毒力基因和耐药性进行检验。结果 VP总检出率为10.56%,以40~49年龄组阳性率最高(17.14%),集中在6~9月份检出,8月份检出率最高(23.17%),不同年龄组、不同月份间检出率差异均有统计学意义(均有P<0.05);79株菌株中O3:K6型74株,占93.67%,79株菌株全部携带不耐热溶血毒素和耐热溶血毒素,未发现有菌株携带耐热性溶血毒素相关的溶血毒素;79株副溶血性弧菌临床分离株中有8株耐药,耐药率为10.13%。其中2株为多重耐药。结论天津地区腹泻患者副溶血性弧菌带菌率较高,且出现超级耐药菌株,应开展污染途径的研究,以便提出防控措施。
Objective To investigate the status, symptom and etiology of vibrio parahaemolyticus (VP) in diarrhea patients in Tianjin. Methods The stool samples and symptoms of diarrhea patients were collected from 7 sentinel hospitals in Tianjin. Vibrio parahaemolyticus was isolated and tested within 24 h. Serotypes, virulence genes and drug resistance of the positive isolates were analyzed. test. Results The positive rate of VP was 10.56%, highest in 40-49 (17.14%), and highest in June-September. The highest detection rate was in August (23.17%), the highest in different age groups (P <0.05). Of the 79 strains, 74 strains were O3: K6 type, accounting for 93.67% of the total, 79 strains were all carrying heat-labile hemolysin and heat-resistant hemolysin, and none The strains were found to carry heat-resistant hemolytic toxin-related hemolysins. Eighty-nine isolates of 79 strains of V. parahaemolyticus were resistant, with a resistance rate of 10.13%. Two of them were multi-drug resistant. Conclusion The prevalence of Vibrio parahaemolyticus in diarrhea patients in Tianjin area is high, and the super-drug-resistant strains appear. Therefore, the research of pollution pathways should be carried out in order to put forward the prevention and control measures.