论文部分内容阅读
目的 观察丙型肝炎患者丙型肝炎病毒(HCV)1b型基因组部分NS5A区核苷酸、氨基酸的变异情况并探讨其与α干扰素疗效的相关性。方法 患者干扰素治疗前、中、后留血标本,用聚合酶链反应(PCR)扩增HCV病毒NS5A区部分基因片段,并用直接测序法测序。与HCVJ株及HCV河北株(HCVHB)比较核苷酸及氨基酸序列的同源性,根据α干扰素疗效分析HCV1b是否存在干扰素敏感决定区。同时分析干扰素治疗是否对HCV基因组变异有影响。结果 患者的HCVNS5A区核苷酸序列比较保守,与HCVJ及HCVHB相比,同源性分别为9822%和9843%,推导的氨基酸序列22092248极少有变异。18例患者经α干扰素治疗,仅4例有效。疗效与氨基酸变异无明确相关。结论 HCV1b型NS5A区核苷酸及氨基酸序列高度保守,目前并未证实存在干扰素敏感决定区。干扰素治疗加速HCVNS5A区核苷酸变异,但对氨基酸变异影响不大。
Objective To investigate the variation of nucleotides and amino acids in NS5A region of hepatitis C virus type 1 genotype 1b hepatitis C virus and to explore its relationship with the efficacy of interferon alfa. Methods Interferon was used to treat the samples before, during, and after the treatment. Part of the NS5A gene of HCV virus was amplified by polymerase chain reaction (PCR) and sequenced by direct sequencing. Nucleotide and amino acid sequence homologies were compared with those of HCVJ strain and HCV strain (HCVHB). According to the efficacy of IFN-α, interferon sensitivity-determining region was analyzed. It is also analyzed whether interferon treatment has an impact on HCV genomic variation. Results The nucleotide sequence of HCV NS5A in patients was relatively conservative. Compared with HCVJ and HCVHB, the homology was 9822% and 9843% respectively, and the deduced amino acid sequence 22092248 was very few. Of the 18 patients treated with interferon alpha, only 4 were effective. No clear correlation between efficacy and amino acid variation. Conclusion The nucleotide and amino acid sequences of HCV NS NSA1 region are highly conserved, and interferon sensitive region has not been confirmed yet. Interferon treatment accelerated nucleotide variation in HCV NS5A region, but had little effect on amino acid variation.