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在动植物細胞染色体的显示方法中,可采用压片法,因为这种方法有很大的优点:操作簡单,节省时間,在切片法中,往往不能得到完整的染色体相,而压片法則能将所包含的染色体,全部保存下来,故較切片法为优。当然,行这种操作,要求却是严格的:第一,器皿必须是很清洁的;第二,所用的試剂、药品要保証化学純粹;第三,时间的掌握和控制要准确。植物方面材料:洋葱根尖。取休眠期之洋葱,放在一个盛满清水的杯口上,使水恰好浸沒洋葱之根部,在室温中放置約3—4日后,洋葱下部卽生出新根,长約2厘米时,剪下固定。固定:剪取长約1厘米左右的根尖,一定要初生根。最好在晚上12时至1时固定,因此时細胞处于分裂最多的时候。固定下述试液:45％冰醋酸或純酒精
In animal and plant cell chromosome display method, the tabletting method can be used, because this method has great advantages: simple operation, save time, in the slicing method, often can not get a complete chromosomal phase, while the tablet Law can contain the chromosomes, all saved, it is better than the slice method. Of course, this operation, the requirements are strict: first, utensils must be very clean; second, the reagents used to ensure chemical purity; Third, the time to master and control should be accurate. Plant material: onion root tips. Take the dormant onion and place it in a cup filled with fresh water, so that the water just submerged the root of the onion. After placing for about 3-4 days at room temperature, the root of the onion produces a new root, about 2 cm long, . Fixed: cut about 1 cm long apical, must be born root. It is best to be fixed between 12 p.m. and 1 p.m., so that the cells are at their most dividing stage. Fix the following test solution: 45% glacial acetic acid or pure alcohol