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目的探讨党参(Dangshen,DS)对间充质干细胞(Mesenchymal stem cells,MSCs)增殖的影响。方法水煮醇沉法制备党参溶液,Ficoll-Paque细胞分离液(1.077×103g/L)分离成人MSCs,体外扩增,将P5~P10代细胞分为DS组和对照组,3d后相差显微镜观察细胞形态,四甲基偶氮噻唑蓝(MTT)微量酶反应法检测细胞代谢活性,流式细胞仪检测细胞周期和免疫表型。结果DS组细胞代谢活性(0.535±0.041),(0.972±0.129)与对照组(0.440±0.066),(0.822±0.097)相比,差异有统计学意义(P<0.05)。DS组S期细胞百分含量(2.14±0.25)%较对照组(1.10±0.04)%增多,凋亡细胞百分含量(1.04±0.62)%较对照组(3.45±1.33)%减少,差异有统计学意义(P<0.05)。DS组细胞CD105和CD166表达阳性,CD34和CD45表达阴性,未观察到形态学改变。结论DS能够促进MSCs的增殖,并且使MSCs仍保持高度的未分化状态。
Objective To investigate the effects of Dangshen (DS) on the proliferation of mesenchymal stem cells (MSCs). Methods Codonopsis solution was prepared by water-alcohol precipitation method. Adult MSCs were isolated from Ficoll-Paque cells (1.077 × 103 g / L) and expanded in vitro. Cells from P5 to P10 were divided into DS group and control group. Cell morphology and MTT assay were used to detect the cell metabolic activity. Cell cycle and immunophenotype were detected by flow cytometry. Results Compared with the control group (0.440 ± 0.066) and (0.822 ± 0.097), the metabolic activity in the DS group (0.535 ± 0.041) and (0.972 ± 0.129) were significantly different (P <0.05). The percentage of S phase cells in DS group was (2.14 ± 0.25)% more than that in control group (1.10 ± 0.04)%, and the percentage of apoptotic cells was (1.04 ± 0.62)% in control group (3.45 ± 1.33)% Statistical significance (P <0.05). The expression of CD105 and CD166 in the DS group was negative, while the expression of CD34 and CD45 was negative. No morphological changes were observed. Conclusions DS can promote the proliferation of MSCs and maintain MSCs in a highly undifferentiated state.