论文部分内容阅读
目的:研究鹰嘴豆芽素A对脂多糖(Lipopolysaccharide,LPS)诱导猪外周血单个核细胞分泌炎性细胞因子的影响。方法:用鹰嘴豆芽素A(50,100μmol/L)处理猪外周血单个核细胞,采用ELISA方法检测其对LPS刺激后细胞分泌肿瘤坏死因子(Tumor necrosis factor-α,TNF-α)、白介素6(Interleukin-6,IL-6)的影响,并用RT-PCR法检测这些细胞因子mRNA的表达,用Western blot法检测核因子κB(Nuclear factorκB,NF-κB)由细胞浆移位至细胞核内情况以判断NF-κB活性变化;同时分别用过氧化物酶体增殖物激活受体(PPAR)α的拮抗剂MK886和PPARγ的拮抗剂GW9662与鹰嘴豆芽素A共同处理猪外周血单个核细胞,观察鹰嘴豆芽素A在PPARα或PPARγ的活性被抑制时对NF-κB活性的影响。结果:鹰嘴豆芽素A能显著降低LPS诱导猪外周血单个核细胞的TNF-α、IL-6的分泌和mRNA的表达;同时鹰嘴豆芽素A显著抑制了LPS诱导猪外周血单个核细胞NF-κB的激活,而此抑制作用均被MK886或GW9662减弱。结论:鹰嘴豆芽素A可通过PPARα和PPARγ抑制LPS诱导猪外周血单个核细胞NF-κB的活化,从而下调LPS诱导的TNF-α、IL-6的生成。
Objective: To study the effects of chickpea sp. A on the secretion of inflammatory cytokines by porcine peripheral blood mononuclear cells (LPS) induced by lipopolysaccharide (LPS). Methods: Peripheral blood mononuclear cells were treated with chickpea sp. A (50 and 100 μmol / L), and the levels of tumor necrosis factor-α (TNF-α), interleukin 6 (Interleukin-6, IL-6). The mRNA expression of these cytokines was detected by RT-PCR. The expression of nuclear factor κB (NF-κB) from the cytoplasm to the nucleus was detected by Western blot To determine the change of NF-κB activity, PBMCs were treated with MK886, a peroxisome proliferator-activated receptor (PPARα) antagonist, and GW9662, an antagonist of PPARγ, The effect of biochanin A on NF-κB activity was observed when PPARα or PPARγ activity was inhibited. Results: Chickpea splenic A significantly decreased the secretion of TNF-α, IL-6 and the mRNA expression of porcine peripheral blood mononuclear cells by LPS. At the same time, chickpea sp. A significantly inhibited LPS-induced porcine peripheral blood mononuclear cells NF-κB activation, and this inhibition were weakened by MK886 or GW9662. Conclusion: Chickpea sp. A can down-regulate the production of TNF-α and IL-6 induced by LPS by inhibiting the activation of NF-κB in porcine peripheral blood mononuclear cells by PPARα and PPARγ.