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目的研究达那唑海藻酸钠微球(DKMG)用于子宫肌瘤动脉栓塞术后细胞凋亡的特点。方法2005年6月至11月于北京协和医院实验动物中心,对24只豚鼠采用雌孕激素负荷法建立子宫肌瘤模型,雌孕激素负荷16周后,随机分为3组:即DKMG栓塞组(DKMG组,8只)、海藻酸钠微球(KMG)栓塞组(KMG组,8只)和未进行子宫动脉栓塞组(对照组,8只)。豚鼠双侧子宫动脉栓塞术在麻醉下开腹直视下进行。于子宫动脉栓塞术4周后,用TUNEL法检测肌瘤细胞凋亡情况,同时用免疫组织化学方法检测肌瘤组织中Bcl-2蛋白的表达。结果DKMG组、KMG组和对照组TUNEL法凋亡细胞比率分别为:(44.6±11.9)%,(33.7±7.3)%和(22.8±9.7)%。DK-MG和KMG组间凋亡细胞比率比较,差异有统计学意义(P(0.05),DKMG组、KMG组和对照组Bcl-2免疫组化染色H评分值分别为:2.7±0.7,2.1±0.3和1.3±0.6。3组间H评分值比较,差异有统计学意义(P(0.05)。结论达那唑海藻酸钠微球用于子宫肌瘤动脉栓塞术可造成细胞凋亡增加,其机制为微球中达那唑药物的局部释放,增加了子宫平滑肌细胞的凋亡比率。
Objective To study the characteristics of danazol sodium alginate microspheres (DKMG) for apoptosis after arterial embolization of uterine fibroids. Methods From June 2005 to November 2008, a total of 24 guinea pigs were treated with estrogen and progesterone load to establish a uterine leiomyoma model in experimental animals center of Peking Union Medical College Hospital. After 16 weeks of estrogen and progesterone loading, they were randomly divided into 3 groups: DKMG embolization group (8 in the DKMG group), KMG (8 in the KMG group), and 8 in the uterine arterial embolism group (control group). Guinea pig bilateral uterine artery embolization under anesthesia open under direct vision. Four weeks after uterine artery embolization, the apoptosis of fibroids was detected by TUNEL method, and the expression of Bcl-2 protein in myoma was detected by immunohistochemical method. Results The percentage of TUNEL-positive apoptotic cells in DKMG group, KMG group and control group were (44.6 ± 11.9)%, (33.7 ± 7.3)% and (22.8 ± 9.7)%, respectively. There was significant difference in apoptotic cell ratio between DK-MG and KMG groups (P <0.05). The scores of Bcl-2 immunohistochemical staining in DKMG group, KMG group and control group were 2.7 ± 0.7 and 2.1 ± 0.3 and 1.3 ± 0.6.3 between groups H score score, the difference was statistically significant (P (0.05) .Conclusion Danazol azadiraphosphate microspheres for uterine fibroids arterial embolization can cause increased apoptosis, The mechanism for local release of danazol drug in microspheres increases the rate of apoptosis of uterine smooth muscle cells.