论文部分内容阅读
目的 研究各种栓塞剂对门静脉分支栓塞后肝脏在形态学上的变化和对肝功能的影响。方法 以 45只大鼠为实验对象 ,除 5只留作对照组外 ,40只分成 4组 ,用 4种不同的栓塞材料进行门静脉右支栓塞 ,术后第 14天和第 2 1天分别处死每组 5只动物 ,剖腹观察肝脏各叶的形态变化 ,然后分离各叶进行称重 ,并计算右叶与全肝的重量比 (比例 1)和肝脏与体重的重量比 (比例 2 )。然后用t检验方法对各组结果进行统计学分析。结果 栓塞后见肝右叶明显变苍白 ,表面呈斑纹状 ,各组之间差异不大 ,在术后 14d及 2 1d ,第 1、2、3组的大鼠肝右叶均见明显变小 ,表面见大小不等淡黄色小瘢痕 ,第 4组小鼠的肝脏形态变化不明显。统计学分析显示 ,碘油酒精 2∶1的高浓度混合液的栓塞作用明显好于碘油酒精 4∶1混合物 (P <0 .0 5 ) ,明胶海绵粉的栓塞作用也十分明显 ,效果与碘油酒精 4∶1混合物相仿 (P>0 .0 5 ) ,但明显好于明胶海绵小块 (P <0 .0 5 )。结论 本实验结果提示 ,门静脉分支的栓塞既可引起该肝叶的萎缩 ,又可促使其他肝叶的代偿性增生 ,门静脉分支栓塞是安全有效的 ,但不同的栓塞剂或栓塞材料有不同的栓塞效果 ,其中碘油酒精 2∶1混合物效果最好
Objective To study the morphological changes of the liver and its effects on liver function after embolization of portal veins by various embolic agents. Methods Forty-five rats were used as experimental subjects. In addition to five rats as control group, 40 rats were divided into 4 groups. Four different embolization materials were used to perform right portal vein embolization. The rats were sacrificed on the 14th and the 2nd day after operation. In each group of 5 animals, the morphological changes of each leaf of the liver were observed by laparotomy, and then each leaf was separated and weighed, and the weight ratio of the right lobe to the whole liver (ratio 1) and the liver to body weight ratio (ratio 2) were calculated. The t-test method was then used for statistical analysis of the results of each group. Results After embolization, the right hepatic lobe showed paleness with a patchy appearance on the surface. There was no significant difference between the groups. On the 14th and 21st days after surgery, the right hepatic lobe of the rats in groups 1, 2 and 3 were significantly smaller. On the surface, small and light yellow scars were seen on the surface, and the liver morphology of the mice in group 4 did not change significantly. Statistical analysis showed that the emulsifying effect of a 2:1 high-dose mixture of lipiodol was significantly better than that of a 4:1 mixture of lipiodol (P<0.05), and the embolization of gelatin sponge powder was also very significant. Lipiodol alcohol 4:1 mixture was similar (P>0.05), but was significantly better than gelatin sponge (P<0.05). Conclusion The results of this experiment suggest that embolization of the portal vein branch can cause both atrophy of the hepatic lobe and compensatory hyperplasia of other liver lobes. Portal vein embolization is safe and effective, but different embolic agents or embolization materials are different. Embolization effect, in which the lipiodol alcohol 2:1 mixture works best