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目的：了解ＳＰＦ级免疫功能正常小鼠及免疫功能缺陷小鼠喉气管粘膜细菌分布情况，探讨菌丛分布与免疫功能的关系。方法：按占群体１５％的比例随机抽取小鼠，无菌手段采集样品作需氧和厌氧培养分离单纯菌落，作出鉴定。结果：免疫功能正常的小鼠有１种：ＢＡＬＢ／ｃ小鼠，检出细菌有赛氏葡萄球菌、模仿葡萄球菌、大肠杆菌；免疫功能缺陷的小鼠有ＢＡＬＢ／ｃ裸小鼠和Ｓｃｉｄ小鼠，检出细菌有：变形杆菌、表皮葡萄球菌、腐生葡萄球菌、赛氏葡萄球菌、大肠杆菌、胶团拟杆菌、口颊拟杆菌、衣氏放线菌、绿脓杆菌、大消化链球菌和有待进一步鉴定的革兰氏阳性杆菌。结论：提示免疫功能正常与缺陷的ＳＰＦ小鼠上呼吸道存在的菌丛可以与小鼠共生，并无引起明显病理改变
OBJECTIVE: To investigate the bacterial distribution of laryngotracheal mucosa in SPF-like immunocompetent mice and immunocompromised mice and to explore the relationship between the distribution of colony and immune function. Methods: Mice were randomly selected according to the proportion of 15% of the population. Samples were collected by aseptic technique for aerobic and anaerobic culture to isolate simple colonies and make identification. Results: There were 1 kinds of mice with normal immune function: BALB / c mice, Staphylococcus aureus, Staphylococcus aureus and Escherichia coli were detected in the BALB / c mice; BALB / c nude mice and Scid mice were immunocompromised Rats, the detection of bacteria are: Proteus, Staphylococcus epidermidis, Staphylococcus saprophytic, Staphylococcus aureus, Escherichia coli, Bacteroides micella, Bacteroides oralis, Actinomyces Chlamydiae, Pseudomonas aeruginosa, Peptostreptococcus And Gram-positive bacilli to be further identified. CONCLUSION: The bacterial flora in the upper respiratory tract of SPF mice with normal and defective immune function can coexist with the mice without causing obvious pathological changes