论文部分内容阅读
小伞山羊草携T型恢复基因的6U染色体片段经自然易位到小麦6AS上,育成了单基因T型恢复系“2114”。携有外缘染色体片段的易位杂合体产生的两种类型配子间存在竞争作用,从而引起易位染色体在配子中传递频率异常。“矮苏3A”/2114的F1自交结实率为91.6%±2.6%。F2群体中株高和育性的分离情况表明,Rht3基因在F2中按单基因分离模式正常分离,而携恢复基因的易位染色体没有按单基因的模式进行分离。以“苏麦3号A”/2114的F1作父本与苏麦3号A测交,根据TCF1群体中可育株和不育株的比例,计算出易位染色体在雄配子中的传递率为30.8%;以苏麦3号A/2114的F1作母本,与不具恢复力的品种“苏麦3号”、“扬麦5号”作父本进行测交,其TCF1群体中可育株和不育株分离比例表明,易位染色体在雌配子中传递正常。同时分析了引起易位染色体在雄配子中传递率低的原因,并提出了利用这条易位染色体的途径,使之在T型恢复系选育中发挥作用。
The small umbrella umbrella goat grass carrying T-type restorer gene 6U chromosome fragment by natural translocation to wheat 6AS, bred single-gene T-type restorer “2114.” The competition between the two types of gametes produced by the translocation heterozygote bearing the outer segment of the chromosome causes the aberrant frequency of translocation chromosomes in gametes. The F1 selfing rate of “dwarf Su 3A” / 2114 was 91.6% ± 2.6%. The segregation of plant height and fertility in F2 population indicated that the Rht3 gene was segregated normally by single gene segregation in F2, whereas the translocated chromosomes carrying the restorer gene were not segregated by the single gene pattern. According to the ratio of fertile plants and sterile plants in TCF1 population, the translocation rate of translocated chromosomes in male gametes was calculated with F1 parent of Sumai No.3 A / 2114 and Sumai No.3. And 30.8% respectively. The male parent of F1 of Sumai No.3 A / 2114 was used as the male parent to restore the phenotype with the non-restoring varieties Sumai 3 and Yangmai 5, The separation ratio of fertile plants and sterile plants shows that the translocations are normal in female gametes. At the same time, the reason of low translocation rate of transposable chromosomes in male gametes was analyzed, and the way of using this translocated chromosomes was proposed to play a role in the breeding of T - type restorer lines.