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铁蛋白不仅存在于正常人肝、脾及骨髓的单核巨噬系统,同时也在粘膜及血细胞等组织中发现。正常情况下,血液有形成分中的铁蛋白很少,但在某些血液病及肿瘤发生时可出现不同程度的铁蛋白增高。因此,可以通过铁蛋白的变化,对某些疾病的早期诊断提供有价值的线索。我们用抗人铁蛋白抗体结合胶体金免疫技术对正常人外周血细胞进行铁蛋白定位观察。取静脉血2—4ml,1000—1500r/min离心10分钟,戊二醛固定后按常规制作电镜标本,半薄
Ferritin not only exists in normal human liver, spleen and bone marrow mononuclear macrophage system, but also in mucosa and blood cells and other tissues found. Under normal circumstances, there are few ferritin in the blood components, but in some hematological diseases and tumors, there may be different degrees of ferritin increase. Therefore, the changes of ferritin can provide valuable clues to the early diagnosis of some diseases. We used anti-human ferritin antibody combined with colloidal gold immuno-technology for normal human peripheral blood cells ferritin localization. Take venous blood 2-4ml, 1000-1500r / min centrifuge for 10 minutes, glutaraldehyde fixed by conventional production of electron microscopy specimens, semi-thin