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目的探讨CSN5在胃癌中的表达及与患者预后的关系。方法运用cBioportal分析工具,分析CSN5在胃癌中基因水平的改变情况;运用qRT-PCR方法检测CSN5 mRNA在永生化的正常胃黏膜细胞株及不同胃癌细胞株中的表达;运用Oncomine数据库(DErrico、Chen胃癌数据集)和TCGA数据库(375例胃癌组织和32例癌旁组织),分析CSN5 mRNA在胃癌组织与正常胃黏膜表达的差异性;运用生存期分析软件Kaplan-Meier plotter,分析CSN5对876例胃癌患者预后的影响;运用Chen胃癌数据集,分析胃癌患者5年生存状态与CSN5表达的关系。结果在胃癌中CSN5主要的基因改变形式为扩增;CSN5 mRNA在多种胃癌细胞株中的表达水平显著高于正常胃黏膜细胞株;CSN5 mRNA在胃癌中的表达显著高于正常胃黏膜。生存分析结果发现,CSN5高表达的患者总生存期较长,CSN5低表达的患者总生存期较短。结论 CSN5在胃癌组织中高表达,且其表达水平与胃癌患者的预后呈正相关,即CSN5高表达的患者,预后较好。
Objective To investigate the expression of CSN5 in gastric cancer and prognosis. Methods The gene level of CSN5 in gastric cancer was analyzed by cBioportal analysis tool. The expression of CSN5 mRNA in immortalized gastric mucosa cell lines and different gastric cancer cell lines was detected by qRT-PCR. Oncomine database (DE Gastric cancer data set) and TCGA database (375 gastric cancer tissues and 32 paracancerous tissues) to analyze the difference of CSN5 mRNA expression in gastric cancer tissue and normal gastric mucosa. Kaplan-Meier plotter was used to analyze the effect of CSN5 on 876 cases Gastric cancer patients prognosis; use of gastric cancer data set to analyze the relationship between gastric cancer 5-year survival status and CSN5 expression. Results The main gene alteration of CSN5 in gastric cancer was amplification. The expression of CSN5 mRNA in gastric cancer cell lines was significantly higher than that in normal gastric mucosa. The expression of CSN5 mRNA in gastric cancer was significantly higher than that in normal gastric mucosa. Survival analysis showed that patients with high CSN5 expression had longer overall survival and patients with low CSN5 expression had a shorter overall survival. Conclusions CSN5 is highly expressed in gastric cancer tissues, and its expression level is positively correlated with the prognosis of gastric cancer patients. That is, patients with high CSN5 expression have a good prognosis.