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以雷公藤不定根为材料,通过在MS培养基中添加不同浓度的天冬氨酸,异亮氨酸,半胱氨酸和精氨酸,研究其对雷公藤不定根生长和次生代谢产物含量的影响。结果表明:第3周添加0.25 mmol·L-1天冬氨酸时,虽然不定根增长量为对照的80%,但不定根及培养液中雷公藤内酯醇含量分别为对照的1.36,1.3倍,雷公藤吉碱含量分别为对照的1.16,1.37倍,雷公藤次碱的含量分别为对照的1.22,1.63倍。第3周添加0.05 mmol·L-1的异亮氨酸时不定根增长量为对照的97.3%,不定根及培养液中雷公藤吉碱分别为对照的1.02,1.27倍,雷公藤次碱的含量分别为对照的1.36,1.15倍。第1周添加0.25 mmol·L-1半胱氨酸时,不定根增长量仅为对照的77.5%,但不定根中雷公藤内酯醇含量为对照的1.87倍。第2周添加1.00 mmol·L-1半胱氨酸时,培养液中雷公藤次碱含量为对照的2.97倍。在第3周精氨酸浓度为0.5 mmol·L-1时,不定根增长量为对照124.2%,雷公藤吉碱和次碱的含量分别为对照的1.3,1.4倍。
Tripterygium wilfordii adventitious root as material, by adding different concentrations of aspartate, isoleucine, cysteine and arginine in MS medium to study its triptolides adventitious root growth and secondary metabolites content influences. The results showed that when 0.25 mmol·L-1 aspartic acid was added in the third week, although the increment of adventitious root was 80% of the control, the content of triptolide in adventitious roots and culture broth was 1.36 and 1.3 times that of the control, The content of basil base was 1.16 and 1.37 times that of the control respectively, and the contents of tripterygium subtilis were 1.22 and 1.63 times of the control respectively. Addition of 0.05 mmol·L-1 isoleucine in the third week resulted in 97.3% increase of adventitious roots, 1.02 and 1.27 times that of Tripterygium wilfordii in adventitious roots and culture medium, respectively The control of 1.36,1.15 times. When 0.25 mmol·L-1 cysteine was added in the first week, the increment of adventitious root was only 77.5% of the control, but the content of triptolide in adventitious root was 1.87 times of the control. When 1.00 mmol·L-1 cysteine was added in the second week, the content of tripterygium in the culture medium was 2.97 times of the control. The arginine concentration was 0.5 mmol·L-1 at week 3, the increment of adventitious root was 124.2% of the control, and the contents of tripterygium and alkali were respectively 1.3 and 1.4 times of the control.