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由真菌Fusarium pseudograminearum引起的小麦冠腐病是澳大利亚小麦生产上最严重的病害。为进行病害抗性鉴定和筛选抗病种质资源,需制备大量的病菌分生孢子。本试验以强致病性菌株FPCS3096为材料,开展了该菌株产孢条件的研究,结果表明:在6种培养基中,以1/2 PDA产孢量最大;在营养生长阶段,在菌丝长满平板后(28℃下需培养6~7 d),延长培养时间(即继续培养),大大促进了后期的产孢,培养12 d的产孢量是培养6 d的4倍,而且在这一阶段,暗培养促进了产孢,连续暗培养的产孢数是连续光培养产孢数的2倍多。试验同时发现,在一定的范围内,培养皿内培养基的重量或平板培养基的厚度对产孢有很大的影响,量越多,产孢量越大,当培养基的重量比为2倍多时,产孢的数量比为7~10倍。与本实验室以前采用的在PDA培养基上培养、然后光照产孢的技术相比,现有的技术将产孢数量提高了20~50倍。
Crown rot disease of wheat caused by the fungus Fusarium pseudograminearum is the most serious disease in wheat production in Australia. For disease resistance identification and screening of disease-resistant germplasm resources, need to prepare a large number of bacteria conidia. In this experiment, the virulent strain FPCS3096 was used as the material to study the sporulation conditions of this strain. The results showed that the sporulation rate of 1/2 PDA was the highest in 6 kinds of culture media. In the vegetative growth stage, After being covered with plate (need to cultivate for 6-7 days at 28 ℃), prolonging the cultivation time (ie continuing to cultivate) greatly promoted sporulation in the later stage. The sporulation amount on 12th day after culture was 4 times of that on 6th day, During this period, dark culture promoted sporulation, and the number of sporulation in continuous dark culture was more than twice that in continuous light culture. The test also found that within a certain range, the weight of culture medium or plate medium culture medium on sporulation has a great impact, the greater the amount of sporulation greater, when the medium weight ratio of 2 Times more, sporozoites than the number of 7 to 10 times. Compared with the technology previously used in our laboratory for culturing on PDA medium and then photospinning, the existing technology increases the number of sporulation by 20 to 50 times.