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目的 探讨外源性一氧化氮（ＮＯ）在顺铂（ＤＤＰ）作用于 ＣＮＥ-２细胞的过程中是否存在增敏效应，为提高鼻咽癌化疗疗效提供实验和理论依据。方法 分别使用不同浓度 ＮＯ供体药物硝普钠（ＳＮＰ）、ＤＤＰ及二者联合干预ＣＮＥ-２细胞，采用硝酸还原酶法检测 ＮＯ的浓度，镜下观察细胞形态学变化，ＣＣＫ-８法检测细胞增殖抑制率以及流式细胞仪检测细胞凋亡。结果 ① ＳＮＰ呈浓度依赖方式抑制 ＣＮＥ-２细胞的增殖、促进凋亡；② 上清液中 ＮＯ浓度与 ＳＮＰ浓度呈正相关，有统计学意义（ｒ＝０．９６８，Ｐ＜０．００１）；③ ＤＤＰ＋ＳＮＰ联合组与 ＳＮＰ组和 ＤＤＰ组相比，ＣＮＥ-２细胞形态学差异显著，漂浮细胞显著增多，贴壁数量逐渐减少并失去原有形态；④ 当 ＳＮＰ６００μｍｏｌ／Ｌ时对细胞增殖无明显影响，然而联合 ＤＤＰ后较单用 ＤＤＰ组细胞抑制率显著提高（ｔ＝９．０４９，Ｐ＜０．００１）；⑤ ＳＮＰ组与ＤＤＰ联用时 ＣＮＥ２细胞凋亡率上升，较单用 ＳＮＰ、ＤＤＰ组显著增强（ｔ＝－１９．８１６，Ｐ＜０．００１；ｔ＝－５．２４２，Ｐ＝０．０３５）。结论 外源性 ＮＯ能抑制 ＣＮＥ-２的增殖，其抑制效应与 ＮＯ浓度呈正相关；合适浓度的外源性 ＮＯ可在对细胞本身不产生明显毒性的情况下显著增强 ＤＤＰ对 ＣＮＥ-２细胞株的化疗敏感性。
Objective To investigate the effect of exogenous nitric oxide (NO) on the proliferation of CNE-2 cells induced by cisplatin (DDP), and to provide experimental and theoretical evidence for improving the efficacy of chemotherapy in nasopharyngeal carcinoma. Methods Nitric oxide sodium (SNP), DDP and their combination were used to treat CNE-2 cells. Nitric acid reductase method was used to detect the concentration of NO. Cell morphology was observed microscopically. CCK-8 assay Cell proliferation inhibition rate and flow cytometry apoptosis. Results ① SNP inhibited CNE-2 cell proliferation and promoted apoptosis in a concentration-dependent manner. ② The concentration of NO in supernatant was positively correlated with the concentration of SNP (r = 0.968, P <0.001). ③ Compared with SNP group and DDP group, the morphology of CNE-2 cells in DDP + SNP combined group was significantly different, the number of floating cells was significantly increased, the number of adherent cells was gradually decreased and the original morphology was lost; ④ When SNP600μmol / L had no effect on cell proliferation (P <0.001). However, the apoptosis rate of CNE-2 cells in SNP group was significantly higher than that in single DDP group when compared with single DDP group (t = 9.049, P <0.001) DDP group was significantly enhanced (t = -19.816, P <0.001; t = -5.242, P = 0.035). Conclusions Exogenous NO can inhibit the proliferation of CNE-2 and the inhibitory effect is positively correlated with the concentration of NO. Exogenous NO at a suitable concentration can significantly enhance the inhibitory effect of DDP on CNE-2 cell line Chemosensitivity.