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观察氟诱导活化后的小胶质细胞ROS和NO的生成状况,为进一步研究氟致中枢神经系统损伤机制提供实验依据。在细胞培养液中加入不同浓度(0、1、5、10、25、50μg/ml)的NaF,培养一定时间后检测小胶质细胞增殖活力、细胞内ROS及培养液NO含量。氟化钠能够增加细胞内ROS含量,50μg/ml NaF处理组,细胞内ROS含量与暴露时间正相关,1、5μg/ml NaF处理组,细胞内ROS含量与暴露时间负相关;BV-2细胞短时间暴露于氟化钠,细胞培养液NO含量与染氟剂量正相关,而随着暴露时间延长,细胞培养液NO含量随染氟剂量升高而降低;随着时间增加,每个NaF处理组NO含量均增加。小胶质细胞活化后导致细胞内ROS、培养液NO含量增加,且与时间、剂量相关。
To observe the generation of ROS and NO after microglial activation induced by fluoride, and to provide experimental evidence for the further study on the mechanism of fluoride-induced central nervous system injury. NaF at different concentrations (0, 1, 5, 10, 25 and 50 μg / ml) were added to the cell culture medium, and the activity of microglial proliferation, intracellular ROS and NO content in the culture medium were detected after a certain period of incubation. Sodium fluoride could increase intracellular ROS content. ROS concentration in 50μg / ml NaF treatment group was positively correlated with exposure time. In 1,5μg / ml NaF treatment group, intracellular ROS content was negatively correlated with exposure time. BV-2 cells In short time exposure to sodium fluoride, the content of NO in cell culture medium was positively correlated with the amount of fluoride in the cells. With the prolongation of exposure time, the content of NO in cell culture medium decreased with the increase of fluoride dose. With the increase of time, Group NO content increased. Activation of microglial cells lead to intracellular ROS, increased NO content in culture medium, and related to time and dose.