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本研究建立了同时测定肉苁蓉中松果菊苷、管花苷A、类叶升麻苷、异类叶升麻苷和2'-乙酰毛蕊花糖苷5种苯乙醇苷类成分含量的一标多测方法(quantitative analysis of multi-components by single marker,QAMS).以松果菊苷为内标,计算其他4种成分相对于该成分的相对校正因子(relative correction factor,RCFs),并对影响RCFs的各种因素进行了考察;然后利用所得RCFs计算各成分的含量,并与外标法测定结果进行比较,以验证一测多评法的可行性和准确性.通过对l0批肉苁蓉药材中5种苯乙醇苷类成分的测定,分别用校正因子法与外标法计算含量,结果发现2种方法测定的结果无显著性差异,证明了采用QAMS测定中药肉苁蓉中苯乙醇苷类成分的含量准确、可行,为肉苁蓉药材的质量控制提供了经济有效的方法.“,”In the present study,a method for the quantitative analysis of multi-components by single marker (QAMS) has been developed and validated for the simultaneous determination of echinacoside (ECH),tubuloside A,acteoside,isoacteoside,and 2'-acetylacteoside in Cistanches Herba.ECH was used as the internal standard (IS) to obtain the relative correction factors (RCFs) of the other four phenylethanoid glycosides (PhGs);meanwhile,various influencing factors on RCFs were investigated under different conditions.The content of each component was calculated with RCF.The results were compared with those obtained by the extemal standard method (ESM) to verify the feasibility and accuracy of the established QAMS method.No significant difference was found in the quantitative results of 10 batches of Cistanches Herba between QAMS and ESM.The proposed QAMS method for simultaneous determination of PhGs in Cistanches Herba is accurate and feasible,providing an efficient and economical approach for the quality control of Cistanches Herba.