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目的:考察藜芦酸在大鼠体内的药代动力学。方法:大鼠口服灌胃给予藜芦酸,在不同时间点采集血样,选择3,4-二羟基苯甲酸甲酯为内标,采用HPLC测定血药浓度,流动相乙腈(A)-1%乙酸(B)梯度洗脱(0~11 min,5%~57%A;11~13 min,57%~5%A),检测波长256 nm,绘制血药浓度-时间曲线,通过Kinetica 4.4软件计算药代动力学参数。结果:灌胃给药后藜芦酸在大鼠体内的达峰时间分别为(45.00±10.00),(31.00±8.22)min,存在二次达峰现象;高、低剂量组的C max分别为(176.85±28.89),(68.14±12.58)mg·L-1,t1/2分别为(87.74±20.74),(227.18±93.78)min,AUC0-∞分别为(50 363.50±11 667.7),(18 830.32±4 354.35)min·mg·L-1,MRT0-∞分别为(201.39±16.72),(227.40±21.37)min。结论:藜芦酸口服吸收较快,消除较慢,体内作用时间较长。
Objective: To investigate the pharmacokinetics of veratric acid in rats. Methods: Rats were orally administered with veratronic acid, blood samples were collected at different time points, and 3,4-dihydroxybenzoic acid methyl ester was selected as internal standard. The plasma concentration was determined by HPLC. The mobile phase consisted of acetonitrile (A) -1% (B) gradient elution (0-11 min, 5% -57% A; 11-13 min, 57-5% A). The detection wavelength was set at 256 nm and the plasma concentration-time curve was plotted by Kinetica 4.4 software Pharmacokinetic parameters were calculated. Results: The peak time of veratronic acid in rats was (45.00 ± 10.00) and (31.00 ± 8.22) min after oral administration, respectively. There was a second peak phenomenon. The Cmax of high and low dose groups were (176.85 ± 28.89) and (68.14 ± 12.58) mg · L-1, respectively. The values of t1 / 2 were (87.74 ± 20.74) and (227.18 ± 93.78) min and AUC0-∞ were (50 363.50 ± 11 667.7) and 830.32 ± 4 354.35) min · mg · L-1, and MRT0-∞ were (201.39 ± 16.72) and (227.40 ± 21.37) min, respectively. Conclusion: Oral absorption of veratronic acid is faster, slower elimination, longer duration of action in vivo.