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目的研究IFN-α-2b对K562细胞黏附功能及黏着斑激酶(FAK)mRNA表达的影响。方法 1.采用Cyto Tox96RNon-Radioactive Cytotoxicity Assay Kit试剂盒检测IFN-α-2b对K562细胞黏附功能的影响。2.流式细胞术检测IFN-α-2b作用于K562细胞前后整合素β1表达水平的变化。3.应用RT-PCR技术检测不同浓度IFN-α-2b对K562细胞FAK mRNA表达水平的影响。结果 1.K562细胞高表达整合素β1。IFN-α-2b可提高K562细胞与纤维黏连蛋白的黏附率,并且可降低整合素β1的表达水平。2.当IFN-α-2b的水平为250万～1 000万U.L-1时,随IFN-α-2b水平的增加FAK mRNA的表达逐渐增高。结论 K562细胞中可能存在整合素β1活化障碍。IFN-α-2b可能是通过增加K562细胞的黏附功能反馈性降低整合素β1的表达水平。IFN-α-2b可能通过增加正常FAK mRNA表达水平来改善β1介导的黏附信号通路的缺陷。
Objective To investigate the effect of IFN-α-2b on adhesion and the expression of focal adhesion kinase (FAK) mRNA in K562 cells. The effect of IFN-α-2b on the adhesion function of K562 cells was detected by Cyto Tox96RNon-Radioactive Cytotoxicity Assay Kit. Flow cytometry was used to detect the changes of integrinβ1 expression in K562 cells treated with IFN-α-2b. The effect of different concentrations of IFN-α-2b on FAK mRNA expression in K562 cells was detected by RT-PCR. Results 1. K562 cells overexpress integrin β1. IFN-α-2b can improve the adhesion of K562 cells to fibronectin and decrease the expression of integrin β1. When the level of IFN-α-2b was 2.5 to 10 million U.L-1, FAK mRNA expression gradually increased with the increase of IFN-α-2b level. Conclusion There may be integrin β1 activation disorder in K562 cells. IFN-a-2b may reduce the expression of integrin β1 by increasing the adhesion function of K562 cells. IFN-α-2b may improve the β1-mediated adhesion signaling pathway by increasing the expression of normal FAK mRNA.