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背景:在银杏叶提取物治疗脑缺血的实验研究中,由于使用全身麻醉药物,有可能诱导脑温改变,影响实验结果准确性。目的:研究常温状态下,国产银杏叶提取物对脑缺血再灌注组织抗氧化酶、脂质过氧化物及脑缺血组织含水量的影响。设计:随机对照的实验研究。地点和材料:实验在华中科技大学同济医学院完成。取24只Wistar大鼠,体质量为250～300g,将实验大鼠随机分为假手术组(n=8)、脑缺血对照组(n=8)、脑缺血银杏叶提取物治疗组(n=8),对照组及治疗组参考Nagasawa方法,制备正常脑温大鼠左侧大脑中动脉缺血2h再灌注2h动物模型,进行对照研究。干预:实验大鼠的脑温用颞肌温度反映,将测温探头包埋入大鼠左侧颞肌深部贴近骨外膜,用半导体氧化物温度传感器连续监测。用60W白炽灯头部加温和自动双控颅脑降温仪调节脑温,使脑温维持在常温(36.5～37℃)水平。按设计方案制备正常脑温大鼠脑缺血再灌注损伤模型,脑缺血银杏叶提取物治疗组大鼠在手术前12,8,4h和脑缺血及再灌注即刻,分别腹腔内注射银杏叶提取物注射液,每次3mL,共5次。脑缺血对照组及假手术组大鼠腹腔内注射相同次数和剂量的生理盐水。主要观察指标:脑缺血组织超氧化物歧化酶、谷胱甘肽过氧化物酶、还原型谷胱甘肽、丙二醛含量及脑缺血组织含水量。结果:脑缺血对照
BACKGROUND: In the experimental study of ginkgo biloba extract in the treatment of cerebral ischemia, due to the use of general anesthetic drugs, it is possible to induce changes in brain temperature and affect the accuracy of the experimental results. Objective: To study the effect of domestic Ginkgo biloba extract on the contents of antioxidant enzymes, lipid peroxides and water content of cerebral ischemic tissue in rats with cerebral ischemia-reperfusion injury. Design: Randomized controlled experimental study. Location and Materials: The experiment was completed at Tongji Medical College, Huazhong University of Science and Technology. Twenty-four Wistar rats weighing 250-300 g were randomly divided into sham-operated group (n=8), cerebral ischemic control group (n=8) and cerebral ischemia-Ginkgo biloba extract treatment group. (n=8) For the control group and treatment group, the Nagasawa method was used to prepare a rat model of normal brain temperature and left middle cerebral artery ischemia for 2 hours and reperfusion for 2 hours. Intervention: The brain temperature of experimental rats is reflected by the temperature of the diaphragm, and the temperature probe is embedded in the deeper part of the left iliac muscle close to the epiphysis, and continuously monitored with a semiconductor oxide temperature sensor. With 60W incandescent lamp head heating and automatic dual control cranial cooling device to adjust the brain temperature, so that the brain temperature is maintained at room temperature (36.5 ~ 37 °C) level. The cerebral ischemia-reperfusion injury model was established in normal brain temperature rats according to the design scheme. The rats in the Gingko biloba leaf extract group were given intraperitoneal injections of Ginkgo biloba at 12, 8, and 4 hours before surgery and immediately after cerebral ischemia and reperfusion. Leaf extract injection, 3mL each time, a total of 5 times. Cerebral ischemia control group and sham operation group rats were injected intraperitoneally with the same number and dose of physiological saline. MAIN OUTCOME MEASURES: Superoxide dismutase, glutathione peroxidase, reduced glutathione, malondialdehyde content, and cerebral ischemia tissue water content in cerebral ischemic tissue. Results: Cerebral ischemia control