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目的对石家庄市水环境中分离到的嗜肺军团菌进行分子分型,研究嗜肺军团菌AFLP分型特征,为建立嗜肺军团菌分子型别库提供资料。方法运用欧洲军团菌感染工作组(European Working Group on Legionella Infection,EWGLI)组织制定的扩增片断长度多态性分型(amplified fragment length polymorphism,AFLP)方法,对石家庄市水环境中分离到的39株嗜肺军团菌进行分子分型,对分型结果进行聚类分析,探讨菌株间的相互关系。结果按照血清分型方法,将水样中嗜肺军团菌分为Lp1型和Lp2~14型。运用AFLP技术对39株嗜肺军团菌菌株分型,其中,将31株Lp1血清型分为18种AFLP型,分别记为AFLP1～AFLP18,以AFLP1型与AFLP11型为主要型别;8株Lp2~14血清型分为7种AFLP型,分别记为AFLPⅠ～AFLPⅦ,以AFLPⅢ为主要型别;在同一地区嗜肺军团菌菌株间,相同血清型别的相似性系数存在差异较大,但仍存在优势株;不同地区之间有部分菌株相似性系数为100%,表明不同地区的水样嗜肺军团菌菌株来源同一性。结论 AFLP分型所得到的不同图谱表明各嗜肺军团菌株基因型呈多态性。
Objective To classify the Legionella pneumophila isolated from the water environment of Shijiazhuang and study the characteristics of AFLP typing of Legionella pneumophila so as to provide information for establishing the molecular type library of Legionella pneumophila. Methods Amplified fragment length polymorphism (AFLP) was used to analyze the genetic diversity of isolated strains in Shijiazhuang from the water environment of Shijiazhuang Legionella pneumophila strain molecular typing, cluster analysis of typing results to explore the relationship between strains. Results According to the method of serotyping, Legionella pneumophila in water samples were divided into Lp1 type and Lp2-14 type. Thirty-nine strains of Legionella pneumophila were genotyped by AFLP technique. Among them, 31 strains of Lp1 serotypes were divided into 18 AFLP types, which were respectively labeled as AFLP1-AFLP18, AFLP1-AFLP11-type as main genotypes. Eight strains of Lp2 ~ 14 serotypes were divided into seven kinds of AFLP type, respectively AFLP Ⅰ ~ AFLP VII, with AFLP Ⅲ as the main type; Legionella pneumophila strains in the same region, the same serotypes similarity coefficient there is a big difference, There existed predominant strains. The similarity coefficient of some strains in different regions was 100%, indicating the origin identity of Legionella pneumophila in different regions. Conclusion The different profiles obtained by AFLP typing showed that the genotypes of Legionella pneumophila strains were polymorphic.