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目的:对采用人类全基因组芯片检测到的甲基丙烯酸环氧丙酯(glycidylmethacrylate,GMA)致人支气管上皮(16HBE)细胞恶性转化过程中差异基因进一步进行其表达改变的研究。方法:采用实时定量PCR技术分析GMA诱导16HBE细胞恶性转化过程中,第10代(转化前期)和第30代(转化后期)细胞的信号转导和有丝分裂相关基因C19orf20、RGS14、EPB49、PSCA、CENPF、CTGF、RASD1的表达。结果:C19orf20、RGS14基因在转化前期细胞中表达分别上调421%、178%;CENPF、CTGF基因在转化后期细胞中表达分别下调93%、77%;EPB49基因在转化前期、转化后期细胞中表达分别上调191%、116%;PSCA基因在转化前期、转化后期细胞中表达分别上调593%、119%;RASD1基因在转化前期细胞中表达上调231%,而在转化后期细胞中表达下调74%。结论:GMA致16HBE细胞恶性转化过程是涉及多基因共同作用的复杂过程,C19orf20、RGS14、EPB49、PSCA、CENPF、CTGF、RASD1基因的异常表达可能在该过程中起着重要作用。
OBJECTIVE: To further investigate the expression of differentially expressed genes in the malignant transformation of human bronchial epithelial (16HBE) cells induced by glycidylmethacrylate (GMA) using human genome-wide arrays. METHODS: Real-time quantitative PCR was used to analyze signal transduction and mitosis-related genes C19orf20, RGS14, EPB49, PSCA, CENPF in the 10th (pre-transformation) and 30th (transformed) phases of GMA-induced malignant transformation of 16HBE cells. Expression of CTGF, RASD1. RESULTS: The expression of C19orf20 and RGS14 genes was up-regulated by 421% and 178% in pre-transformation cells, and CENPF and CTGF genes were down-regulated by 93% and 77% respectively in cells at the late stage of transformation. EPB49 genes were expressed in cells at the early and late stages of transformation. Up-regulation was 191%, 116%; PSCA gene expression was up-regulated in pre-transformation and post-transformation cells by 599.3% and 119%, respectively; RASD1 gene was up-regulated by 231% in pre-transformation cells and down-regulated by 74% in cells after transfection. CONCLUSION: The malignant transformation of 16HBE cells induced by GMA is a complex process involving multiple genes. The abnormal expression of C19orf20, RGS14, EPB49, PSCA, CENPF, CTGF and RASD1 genes may play an important role in this process.