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AIM To analyze the association of HLA-DRB1 withautoimmune hepatitis(AIH)in patients from China.METHODS In 32 patients and 48 healthy controls,polymerase chain reaction amplification with sequence-specific primers(PCR-SSP)was performed to examine theassociation of certain alleles or polymorphic sequences ofHLA-DRB1 with AIH.RESULTS HLA-DRB1 typing by PCR-SSP showed that DR4had a significantly increased frequency among patientswith AIH versus healthy control(46.9% versus 20.8%;relative risk=3.35,P=0.014).In subtypes of DR4,therewas a trend of increase in the gene frequency of DRB1~*0405 in patients with AIH versus healthy controls(21.9%vs 6.3%,P=0.04,but P_c=0.08).In addition,asignificant increase was found in the alleles frequencyencoding QRRAA from the third hyperpolymorphic regionof DR4 in the patients with AIH(86.7% of DR4 positivepatients vs 40.0% in DR4 positive controls,P=0.016,P_c=0.028,RR=9.75).CONCLUSION AIH in Chinese is associated with HLA-DR4.There is a relationship between QRRAA sequencewithin the third hyperpolymorphic region of the DRB alleleand AIH in Chinese.
AIM To analyze the association of HLA-DRB1 withautoimmune hepatitis (AIH) in patients from China. METHODS In 32 patients and 48 healthy controls, polymerase chain reaction amplification with sequence-specific primers (PCR-SSP) was performed to examine the association of certain alleles or polymorphic sequences of HLA-DRB1 typing by PCR-SSP showed that DR4had a significantly increased frequency among patients with AIH versus healthy control (46.9% versus 20.8%; relative risk = 3.35, P = 0.014) .In subtypes of DR4, there was a trend of increase in the gene frequency of DRB1 ~ * 0405 in patients with AIH versus healthy controls (21.9% vs 6.3%, P = 0.04, but P_c = 0.08) .In addition, asignificant increase was found in the alleles frequencyencoding QRRAA from the third hypermorphic region of DR4 in the patients with AIH (86.7% of DR4 positivepatients vs 40.0% in DR4 positive controls, P = 0.016, P_c = 0.028, RR = 9.75) .CONCLUSION AIH in Chinese is associated with HLA- DR4.There is a relationship between QRRAA sequence with the third hyperpolymorphic region of the DRB allele and AIH in Chinese.