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本文用儿茶酚胺合成酶之一的酪氨酸羟化酶(TH)抗体,用不标记抗体过氧化物酶——抗过氧化物酶(PAP)法包埋前免疫电镜技术观察了金黄地鼠松果体内的单胺类神经分布.结果发现松果体内有TH样免疫反应阳性神经纤维和TH样兔疫反应阳性神经元.TH样免疫反应阳性的经元散在于松果体实质内.其细胞体呈卵圆形或梭形,最大直径为15～20μm,具有一个或两个短的细胞突起.细胞核大,呈圆形.细胞质内有线粒体,核糖体和高尔基复合体.TH样免疫反应阳性神经元与松果体细胞体、松果体细胞突起、TH免疫反应阳性或阴性神经终末相接触,但无明显的膜特化的结构.在TH免疫反应阳性神经终末内有许多颗粒小泡和无颗粒小泡.TH免疫反应阳性神经纤维与松果体细胞相接触,但无明显的膜特化结构.
In this paper, tyrosine hydroxylase (TH) antibody, one of the catecholamine synthetases, was used to observe the effect of golden hamster The results showed that the TH-like immunoreactive positive nerve fibers and TH-like immunoreactive neurons were found in the pineal gland.THE-like immunoreactivity was found in the pineal parenchyma.The cells The body has an oval or fusiform shape and has a maximum diameter of 15 to 20 μm and has one or two short cell protrusions. The nucleus is large and round, with mitochondria, ribosomes and Golgi complexes in the cytoplasm. The TH-like immunoreactivity Neurons contacted with pineal somatic cell bodies, pineal somatic cells, TH immunoreactive positive or negative terminal nerves, but without obvious membrane-specific structures. There were many small particles in the TH immunoreactive positive terminal nerve Bubble and no-particle vesicles.TH immunoreactive positive nerve fibers were in contact with pineal cells, but no obvious membrane-specific structure.