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目的探讨石杉碱甲(huperzine A)改善急性低压低氧模型大鼠的空间记忆能力以及对模型大鼠脑组织氧化应激损伤的缓解作用。方法 48只大鼠随机分为4组:平原组(对照组)、平原给药组(0.1 mg.kg-1)、高海拔组(模拟6 000 m海拔)、高海拔给药组。比较各组大鼠Morris水迷宫学习与记忆测试成绩及大脑海马组织中谷胱甘肽、丙二醛含量和过氧化氢酶、超氧化物歧化酶、乳酸脱氢酶的活力。结果与高海拔组比较,高海拔给药组大鼠逃避潜伏期明显较短(P<0.05),60 s内穿越平台次数明显较多(P<0.05),目标象限滞留时间明显较长(P<0.05),海马组织中谷胱甘肽含量、超氧化物歧化酶、过氧化氢酶活力明显较高(P<0.05),丙二醛含量、乳酸脱氢酶活力明显较低(P<0.05),而高海拔给药组与平原对照组比较差异不显著。结论石杉碱甲对急性低压低氧导致的大鼠脑组织氧化应激损伤具有缓解作用,同时改善模型大鼠的空间记忆能力。
Objective To investigate the effects of huperzine A on improving the spatial memory of rats with acute hypobaric hypoxia and the protective effect of huperzine on the oxidative stress in the rat brain. Methods Forty-eight rats were randomly divided into 4 groups: plain group (control group), plain group (0.1 mg.kg-1), high altitude group (simulated 6 000 m altitude) and high altitude group. The Morris water maze learning and memory test scores and the glutathione and malondialdehyde content, catalase, superoxide dismutase and lactate dehydrogenase activity in hippocampus of rats in each group were compared. Results Compared with the high altitude group, the escape latency of rats in the high altitude group was significantly shorter (P <0.05), and the number of passing through the platform in 60 s was significantly higher (P <0.05) and the retention time in the target quadrant was significantly longer (P < 0.05). The content of glutathione, the activity of superoxide dismutase and catalase in hippocampus were significantly higher (P <0.05), the content of malondialdehyde and the activity of lactate dehydrogenase were significantly lower (P <0.05) However, there was no significant difference between high altitude group and plain control group. Conclusion Huperzine A can relieve oxidative stress injury induced by acute hypobaric hypoxia in rats and improve the spatial memory ability of the model rats.