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【目的】通过喉狭窄瘢痕组织的病理学研究,了解炎症细胞在狭窄瘢痕组织表达及意义。【方法】垂直喉部分切除术后2～3月出现喉狭窄10例和未狭窄16例喉瘢痕肉芽组织为实验组及10例正常声带组织为对照组,分别进行苏木精-伊红及免疫组化染色,观察组织切片中中性粒细胞、巨噬细胞、T淋巴细胞表达,并在全自动图像分析系统下进行细胞计数。【结果】喉狭窄瘢痕组织与非狭窄喉瘢痕组织、正常声带比较,固有层炎症细胞大量聚集,主要以巨噬细胞和T淋巴细胞为主。比较非狭窄喉瘢痕组织及正常声带组织,喉狭窄瘢痕组织的中性粒细胞、巨噬细胞、T淋巴细胞数量均明显增加,差异有统计学意义(P<0.01)。【结论】喉狭窄瘢痕肉芽组织中存在明显炎症反应,持续炎症反应可能是导致喉狭窄瘢痕肉芽增生重要因素之一。
【Objective】 To investigate the expression and significance of inflammatory cells in the sarcoma of the scar through the pathological study of the scar tissue in the larynx. 【Methods】 In the period of 2-3 months after vertical partial laryngectomy, 10 cases of laryngeal stenosis and 16 cases of laryngeal scar were selected as the experimental group and 10 cases of normal vocal cords as the control group. Hematoxylin and eosin and immunization Tissue staining was performed to observe the expression of neutrophils, macrophages and T lymphocytes in tissue sections. The cell counts were performed under an automatic image analysis system. 【Results】 Compared with the normal vocal cords, there were a large number of inflammatory cells in the lamina propria, mainly macrophages and T lymphocytes. The number of neutrophils, macrophages and T lymphocytes in non-stenotic laryngeal scar tissues and normal vocal cords and laryngeal stenosis scar were all significantly increased, with statistical significance (P <0.01). 【Conclusions】 There is a significant inflammatory reaction in laryngeal stenosis and granulation tissue. Continuous inflammatory reaction may be one of the important factors that cause granulation proliferation in laryngeal stenosis scar.