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以金针菇为试材,以菌丝体干重和菌丝球数目为考察指标,选择碳源、氮源、无机盐、维生素为影响因素,通过发酵试验和正交实验确定最佳液体种子培养基。结果表明:金针菇液体培养的最佳碳源为可溶性淀粉,最佳氮源为黄豆粉,最佳无机盐为KH2PO4,最佳维生素为VB2;最佳培养基配方为4%可溶性淀粉、5%黄豆粉、0.05%KH2PO4、0.0075%VB2。
Taking Flammulina velutipes as test material, taking the dry weight of mycelium and the number of mycelium as the index, the optimum liquid seed medium was determined by fermentation and orthogonal experiments with carbon source, nitrogen source, inorganic salt and vitamins as the influencing factors . The results showed that the optimum carbon source for Flammulina velutipes liquid culture was soluble starch, the best nitrogen source was soybean flour, the best inorganic salt was KH2PO4 and the best vitamin was VB2. The best medium was 4% soluble starch, 5% soy bean Powder, 0.05% KH2PO4, 0.0075% VB2.