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目的 :探讨心肌缺血再灌注损伤 ( MIRI)时乳酸脱氢酶 ( L DH)活性的变化以及中医药的保护作用机制。方法 :实验兔 30只 ,随机分为对照组、盐水组和葛根素葡萄糖注射液 ( PGI)组 ,每组 10只。制作 MIRI模型 ,观察血清及心肌组织 L DH活性、血栓素 B2 ( TXB2 )和 6酮前列腺素 F1α( 6 keto PGF1α)含量、TXB2 /6 keto PGF1α( T/ K)比值变化及 PGI对它们的影响。结果 :MIRI过程中 ,盐水组血清 L DH活性进行性升高( P<0 .0 5或 P<0 .0 1) ,心肌组织内 L DH活性明显下降 ( P<0 .0 1)而 T/ K比值显著增高 ( P<0 .0 1) ;PGI能降低血清中升高的 L DH活性 ,降低心肌组织中 L DH活性和 T/ K比值 ,均有显著性差异 ( P<0 .0 5和 P<0 .0 1)。结论 :PGI可通过纠正 TXA2 与前列环素的平衡 ,对 MIRI时 L DH活性的异常改变起积极的调节作用 ,以达到心肌保护作用
Objective: To investigate the changes of lactate dehydrogenase (LDH) activity during myocardial ischemia-reperfusion injury (MIRI) and the protective mechanism of traditional Chinese medicine. Methods: Thirty rabbits were randomly divided into control group, saline group and puerarin glucose injection (PGI) group, 10 in each group. MIRI model was established to observe serum and myocardial LDH activity, thromboxane B2 (TXB2) and 6 keto prostaglandin F1α (6 keto PGF1α) content, TXB2 / 6 keto PGF1α (T/K) ratio change, and PGI influence on them. . RESULTS: During the MIRI process, serum LDH activity in the saline group increased progressively (P<0.05 or P<0.01), and LDH activity in the myocardial tissue was significantly decreased (P<0.01). The ratio of /K was significantly higher (P<0.01); PGI could reduce the elevated LDH activity in serum and decrease the LDH activity and T/K ratio in myocardial tissue, with significant differences (P<0. 0 5 and P<0. 0 1). Conclusion: PGI can positively regulate abnormal changes of LDH activity in MIRI by correcting the balance between TXA2 and prostacyclin to achieve myocardial protection.