Interaction Between HMG Proteins (1+2) and the Negative Regulatory Region 1(NCR1) in the 5'-fla

• 作 者: 钱若兰 陈雅娣 胡玉龙 李民乾 王中怀 徐耀良
• 机 构: Shanghai Institute of Cell Biology, Academia Sinica, Shanghai 200031, PRC,Shanghai Institute of Cell
• 出 处: Science in China,Ser.B
• 发表日期: 1993年08期
• 关键词: globin NCR1 in the 5 Proteins helix folded handed DNA circular inducing

The pattern of high mobility group proteins 1 and 2 (HMG1,2) interaction with the 5’-flanking sequence of the human β-globin gene has been analyzed by scanning tunnelling microscopy (STM). A 200 bp negative regulatory region in the 5’-flanking sequence of the human β-globin gene can be folded by HMG proteins 1 and 2 into a circular structure (diameter 70±6) with a linear tail which seems to be a left-handed double helix structure.

The pattern of high mobility group proteins 1 and 2 (HMG1,2) interaction with the 5’-flanking sequence of the human β-globin gene has been analyzed by scanning transduction microscopy (STM). A 200 bp negative regulatory region in the 5 -flanking sequence of the human β-globin gene can be folded by HMG proteins 1 and 2 into a circular structure (diameter 70 ± 6) with a linear tail which seems to be a left-handed double helix structure.