论文部分内容阅读
目的研究17β-雌二醇对涎腺黏液表皮样癌高转移细胞Mc3黏附、侵袭和移动力的作用。方法采用细胞体外黏附实验、体外移动实验、化学趋化性实验以及明胶底物SDS-PAGE凝胶电泳对基质金属蛋白酶(MMP)活性测定的方法研究17β-雌二醇对Mc3细胞黏附、侵袭和移动力可能存在的作用,用免疫组化的方法检测Mc3细胞的雌激素受体的表达。结果不同浓度(10-9、10-8、10-7、10-6mol/L)的17β-雌二醇处理Mc3细胞72h后,其黏附率分别为38·3%、50·4%、69·2%、91·1%;而对照组为25·0%;作用48h后,10-9、10-8、10-7、10-6mol/L的17β-雌二醇对Mc3细胞在纤维连接蛋白(FN)上的移动促进率分别为16·9%、40·9%、36·4%、38·8%。在10-6mol/L的17β-雌二醇的作用下,其对FN的化学趋化性增加60·3%。10-9、10-8、10-7、10-6mol/L的17β-雌二醇均可不同程度的促进Mc3细胞基质金属蛋白酶(MMP-2)的活性,在10-6mol/L时尤为显著。在Mc3细胞中有雌激素核受体的表达。结论生理浓度的雌激素可促进Mc3细胞的黏附、侵袭和移动力。
Objective To investigate the effect of 17β-estradiol on adhesion, invasion and migration of mucoepidermoid carcinoma cell line Mc3 in salivary gland. Methods The adhesion and invasion of 17β-estradiol to MC3 cells were studied by in vitro cell adhesion assay, in vitro migration assay, chemotaxis assay and gelatin substrate SDS-PAGE gel electrophoresis to determine the activity of matrix metalloproteinase (MMP) The possible role of motility, the use of immunohistochemical detection of Mc3 cells estrogen receptor expression. Results After treated with 17β-estradiol at different concentrations (10-9, 10-8, 10-7, 10-6mol / L) for 72h, the adhesion rates of Mc3 cells were 38.3%, 50.4%, 69 · 2%, 91 · 1%, while the control group 25.0%; 48h, 10-9,10-8,10-7,10-6mol / L of 17β-estradiol on Mc3 cells in the fiber The rate of movement promotion on connexin (FN) was 16.9%, 40.9%, 36.4% and 38.8% respectively. Under the action of 10-6mol / L 17β-estradiol, chemotaxis to FN increased by 60.3%. 10-9,10-8,10-7,10-6mol / L 17β-estradiol can promote the activity of matrix metalloproteinase (MMP-2) of Mc3 cells to different degrees, especially in the 10-6mol / L Significant. Expression of estrogen nuclear receptors in Mc3 cells. Conclusion Physiological concentrations of estrogen can promote the adhesion, invasion and migration of Mc3 cells.