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通过设计实验与分析模型,研究相干反斯托克斯拉曼散射成像对于样品尺寸小于系统点扩展函数尺寸的情况,分析相干反斯托克斯拉曼散射图像周围的depth的形成原因.分析过程首次引入轴向传输动态位移光线(此光线由相干体积元内轴心光线抽象出)对球形或柱形样品直径小于系统点扩展函数横向与轴向尺寸进行建模解析,对于所建模型定量分析结果表明,对于样品尺寸小于系统点扩展函数尺寸样品折射率调制了有效作用深度.Gouy相移只是其表观现象,是一个伴随性特征,因为物理学中存在的另一个极端,当样品尺寸足够小且具有相当的折射率,Gouy相移的作用近似为0,此时,样品周围的depth主要与样品的折射率及系统相干层析体积元内有效作用长度有关,相干反斯托克斯拉曼散射参量信号,具有自身的波矢匹配条件,不仅有大小,有方向,而且还受到样品自身材料折射率的影响比较明显.也就是对于参量过程波矢匹配条件是因,Gouy相移是伴随性特征.自此,通过相干反斯托克斯拉曼散射间接说明所用的紧聚焦参量信号成像过程,正是因为波矢匹配条件的存在使二次信号继承了激发光的紧聚焦特性,而拥有了继承性的Gouy相移,结合实验结果采用物理学的极端假设表明,Gouy相移不是产生depth的主要原因,主要原因是样品及周围的环境的折射率与系统相干层析体积元内有效作用长度之间共同作用的结果.而实验结果与模型定量分析的结果相吻合,此实验帮我们找到了影响CARS图像样品周围depth的成因机制,对于其他小尺寸样品的参量成像结果的分析具有一定的借鉴意义.通过设计实验,结合定量模型分析,首次明确造成小尺寸样品CARS图像周围的depth的真正原因,此抽象模型的拓展性对纳观参量过程的分析具有得天独厚的优势.抽象出的主能量光线动态位移模型成功分析纳观成像结果表明,相干作用长度之内有效作用长度及其行进路径的过程论的主因分析方法是研究纳观作用机制的最佳方法.“,”This paper investigates coherent conditions for smaller anti-Stokes Raman scattering imaging sample size than the system point spread function size and the reasons for depth around coherent anti-Stokes Raman scattering images using experimental design and analysis models . The axial transmission dynamic displacement light method ,which subtracts axial light from the coherent volume element ,is introduced to model and analyse lateral and axial dimensions for spherical or cylindrical samples with a smaller diameter than the system point spread function .The Gouy phase shift effect was approximately zero for small sample size and large refractive index .The main reason was the interaction between sample refractive index and the surrounding environment , and the system coherent tomographic volume element effective length . The obtained results apply only to CARS image analysis ,where the sample size is smaller than the system point spread function ,but it is also the first paper that clarifies the underlying reason for depth around CARS images using design experiments and quantitative model analysis .Successful nano-imaging analysis using axial transmission dynamic displacement light verified that the nano-action mechanism is similar to coherent action length ,effective action length and its travel path .