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结核分支杆菌对抗结核药物产生耐受性，已成为全球结核防治工作面临的一个严重问题［１，２］。传统的药敏试验需１～２个月，难以满足临床诊治的需要。近年来发展的ＢＡＣＴＥＣ法测定耐药性也需要３～４周，仍为生长依赖性，且需昂贵仪器设备，因而限制其推广应用。因此对结
Mycobacterium tuberculosis resistance to tuberculosis has become a global tuberculosis prevention and control work is facing a serious problem [1,2]. The traditional drug susceptibility test takes 1 ~ 2 months, it is difficult to meet the needs of clinical diagnosis and treatment. In recent years, the development of BACTEC assay of drug resistance also takes 3 to 4 weeks, is still growth-dependent, and require expensive equipment, thus limiting its promotion. So on the knot