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目的:研究Egr-1基因在2种腺癌细胞A549和Hela放射前后的表达变化及对放射敏感性的影响。方法:培养肺腺癌A549细胞和宫颈腺癌Hela细胞,分别提取4 GyX射线照射前及照射后不同时间点的细胞的总RNA行荧光定量PCR(FQ-PCR)检测Egr-1表达水平;收获4 GyX射线照射前及照射后不同时间点的细胞处理行流式细胞术检测其凋亡;对照射不同剂量的细胞继续培养10-14天,进行克隆形成计数,计算克隆形成率及存活分数。结果:FQ-PCR结果显示,放射后Egr-1基因表达水平在2种细胞中均明显升高且于放射后1 h达到峰值,A549细胞的峰值明显高于Hela细胞;流式细胞术检测结果显示,A549细胞凋亡明显高于Hela细胞;克隆形成实验结果显示,A549细胞存活分数明显低于Hela细胞。结论:Egr-1基因在不同腺癌细胞表达水平不同并影响其放射敏感性。
OBJECTIVE: To study the expression changes of Egr-1 gene before and after radiotherapy in two kinds of adenocarcinoma cell lines A549 and Hela and its effect on radiosensitivity. Methods: The lung adenocarcinoma A549 cells and cervical adenocarcinoma Hela cells were cultured. The total RNA of cells before and after 4 Gy X-ray irradiation was extracted and the expression of Egr-1 was detected by fluorescence quantitative PCR (FQ-PCR) The cell apoptosis was measured by flow cytometry before 4 Gy X-ray irradiation and at different time points after irradiation. The cells were irradiated with different dosages and cultured for 10-14 days. The colony formation rate and the survival rate were calculated. Results: The results of FQ-PCR showed that the expression of Egr-1 gene was significantly increased in both of the two kinds of cells after radiation and peaked at 1 h after irradiation, and the peak value of A549 cells was significantly higher than that of Hela cells. The results of flow cytometry The apoptosis of A549 cells was significantly higher than that of Hela cells. The clone formation assay showed that the survival rate of A549 cells was significantly lower than that of Hela cells. Conclusion: The expression of Egr-1 gene is different in different adenocarcinoma cells and affects its radiosensitivity.