Seven amino acid peptide mimic from HVR1 of HCV influences cytokine profile of immune cells

• 作 者: Song Chunhui Chen Liming Yang Bin Chi Shuping Cheng Yun
• 机 构: Department of Liver Disease Research Center for Biology, No. 302 Hospital of PLA ,Beijing 100039, Ch
• 出 处: 中国人民解放军军医大学学报（英文版）
• 发表日期: 2010年5期
• 关键词: Seven amino acids peptide mimic Interleukin-10 Interferon-γ Tumor necrosis facto

Objective:To investigate the actions of cytokine profile in the immune cells by a seven amino acid peptide mimic from HVR1 of HCV (GQTYTSG, named 71). Methods: The peripheral blood mononuclear cells (PBMCs) from 17 healthy blood donors were stimulated with 7P, and the cytokine levels in CD4+CDS+,CD4+CD8+ T cells, NK, NKT ceils were analyzed by the intracellular cytokine staining. Results: The frequency of cells which secreted interferon-y (IFN-γ) was found to be significantly increased in all cells, interleukin-10(IL-10) was significantly increased in CD4+CD8-, CD4- CD8+ T cells but decreased in NK, 7P could induce a cytokine profile in different immune cells in vitro and there was some difference between the CD4+CD8- ,CD4- CD8+ T cells which represented the adaptive immunity ceils and NK, NKT cells which represented the innate immunity cells.This kind of variation of cytokine profile might contribute to anti-virus and anti-inflammatory immune reaction.