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用三株单克隆抗体(McAb):T_4、T_8和HI_(43)研究不同剂量~(60)Coγ线照射对人外周血淋巴细胞亚群的辐射效应;玫瑰花环法分离T、B淋巴细胞;T_4McAb标记淋巴细胞,经细胞亲和层析得到T_4~+和非T_4~+(即去除了T_4~+亚群的淋巴细胞)细胞。部分T、B、T_4~+和非T_4~+细胞接受10Gy~(60)Coγ线照射,随后各种细胞匹配分组配养,以~3H-TdR掺入法观察PWM对淋巴细胞及其亚群的激活功能及(60)Coγ线的辐射效应。结果表明:T_4~+、T_8~+和HI_(43)~+细胞受到0.1Gy(60)Coγ线照射后即刻(1小时之内)间接免疫荧光试验就可呈现非常显著的辐射效应,T_8~+和HI_(43)~+的辐射敏感性高于T_4~+亚群。T、B淋巴细胞均可被PWM所激活,后者强于前者;PWM对B和非T_4~+细胞激活作用相当,唯T_4~+细胞最弱。T细胞和T_4~+细胞与B细胞均有协同作用。前者与B细胞的协同作用更大。当T、T_4~+细胞或B细胞受到10Gy(60)Coγ线照射后,它们之间的协同作用消失。非T_4~+细胞与B细胞既无协同作用也无抑制作用。受10Gy(60)Coγ线照射后,T_4~+亚群~3H-TdR掺入CPM减少没有B和非T_4~+细胞严重,后二者差别无显著性。
Three monoclonal antibodies (McAbs): T_4, T_8 and HI_ (43) were used to study the radiation effects of different doses of ~ (60) Co γ radiation on human peripheral blood lymphocyte subsets; rosette was used to separate T and B lymphocytes; T_4McAb labeled lymphocytes, T_4 ~ + and T_4 ~ + (that is, T_4 ~ + subpopulations removed lymphocytes) cells were obtained by cytophilic chromatography. Some T, B, T_4 ~ + and non-T_4 ~ + cells were irradiated with 10Gy ~ (60) Coγ ray, and then all kinds of cells were matched and grouped. The effects of PWM on lymphocytes and their subpopulations Activation function and (60) Coγ line radiation effect. The results showed that the indirect immunofluorescence test showed that T_4 ~ +, T_8 ~ + and HI_ (43) ~ + cells could show a very significant radiation effect immediately after (1 hour) irradiation with 0.1Gy (60) + And HI_ (43) ~ + were higher than that of T_4 ~ + subpopulations. T and B lymphocytes were all activated by PWM, while the former was stronger than the latter. The activation of PWM on B and non-T 4 + cells was similar, but the weakest of T 4 + cells. T cells and T_4 ~ + cells and B cells have synergistic effects. The former is more synergistic with B cells. When T, T 4 + cells or B cells were irradiated with 10 Gy (60) Co gamma rays, the synergy disappeared. Non-T_4 ~ + cells and B cells neither synergistic nor inhibitory effect. After 10Gy (60) Coγ ray irradiation, the incorporation of 3H-TdR into T_4 ~ + subpopulation decreased, and there was no significant difference between the two groups except for B and non-T_4 ~ + cells.