论文部分内容阅读
AIM: To evaluate the effects of abdominal trauma on hemorrhagic shock-induced acute lung injury in rats. METHODS: Five groups were allocated (n = 8) in the study. GroupⅠwas taken as the control group, groupⅡas the hemorrhagic shock group, groupⅢas hemorrhagic shock + laparotomy, groupⅣas hemorrhagic shock + splenectomy and groupⅤas splenec-tomy + omentectomy + hemorrhagic shock group. Hemorrhagic shock was induced by drawing blood and reducing mean arterial pressure (MAP) to 40 mmHg within 10 min. After a hypotensive period of 1 h, animals were resuscitated. Bronchoalveolar lavage (BAL) was performed to recover cells from the alveolar space with 40 ml of BAL fluid after resuscitation malondialdehyde (MDA) and L-γ-glutarnyl-L-cysteinyl-glycine (GSH) levels were measured in serum, eryth-rocytes and lung tissue. RESULTS: Serum, erythrocyte, lung tissue MDA and GSH levels were significantly increased in hemorrhagic shock groupsⅡ-Ⅴ(P < 0.05). Lymphocyte, neutrophil and alveolar macrophage counts in BAL fluid indicated a significant difference between control and shock groups (P < 0.05). CONCLUSION: The degree of trauma increases hemorrhagic shock-induced acute lung injury.
AIM: To evaluate the effects of abdominal trauma on hemorrhagic shock-induced acute lung injury in rats. METHODS: Five groups were allocated (n = 8) in the study. Group Ⅰ was taken as the control group, group Ⅱ as the hemorrhagic shock group, group Ⅲ as hemorrhagic shock + laparotomy, groupⅣas hemorrhagic shock + splenectomy and groupⅤas splenec-tomy + omentectomy + hemorrhagic shock group. Hemorrhagic shock was induced by drawing blood and reducing mean arterial pressure (MAP) to 40 mmHg within 10 min. After a hypotensive period of 1 h , animals were resuscitated. Bronchoalveolar lavage (BAL) was performed to recover cells from the alveolar space with 40 ml of BAL fluid after resuscitation of malondialdehyde (MDA) and L- [gamma] -glutarnyl-L-cysteinyl-glycine serum, eryth-rocytes and lung tissue. RESULTS: Serum, erythrocyte, lung tissue MDA and GSH levels were significantly increased in hemorrhagic shock groupsⅡ-Ⅴ (P <0.05). Lymphocyte, neutrophil and alveol ar macrophage counts in BAL fluid indicated a significant difference between control and shock groups (P <0.05). CONCLUSION: The degree of trauma increases hemorrhagic shock-induced acute lung injury.