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目的:检测大肠癌及大肠腺瘤组织中miR-451及巨噬细胞游走抑制因子(macrophage migration inhibitory factor,MIF)的表达水平,并分析其与大肠癌相关临床病理因素的关系。方法:收集25对大肠癌组织和大肠癌旁组织,10例大肠腺瘤组织,应用RT-PCR方法检测miR-451的表达水平(以U6为对照),应用免疫组化检测MIF蛋白的表达情况,并分析二者与大肠癌相关临床病理因素的关系。结果:miR-451在大肠癌及大肠腺瘤组织中明显下调(P<0.01,P<0.05)。其中在大肠癌组织中上调6例,下调19例,下调比率76%(19/25)。在大肠腺瘤组织中上调3例,下调7例,下调比率70%(7/10)。MIF蛋白在大肠癌组织中阳性表达18例,阳性率72%,在大肠腺瘤组织中阳性表达5例,阳性率50%;癌旁组织阳性表达8例,阳性率32%,差异有统计学意义(P<0.05),并且miR-451与MIF表达水平与大肠癌的分化相关(P<0.05),与年龄、性别、Dukes分期、组织类型、浸润深度、淋巴结转移等无明显相关。结论:miR-451在大肠癌组织及大肠腺瘤组织中低表达,可能通过抑制MIF负向调控了大肠癌的发生和发展。
OBJECTIVE: To detect the expression of miR-451 and macrophage migration inhibitory factor (MIF) in colorectal carcinoma and colorectal adenoma and to analyze its relationship with clinicopathological factors related to colorectal cancer. Methods: Twenty-five pairs of colorectal cancer tissues and colorectal cancer tissues and 10 colorectal adenomas tissues were collected. The expression of miR-451 was detected by RT-PCR (U6 as control), and the expression of MIF protein was detected by immunohistochemistry , And analyze the relationship between them and the clinicopathological factors related to colorectal cancer. Results: miR-451 was significantly down-regulated in colorectal carcinoma and colorectal adenoma (P <0.01, P <0.05). Among them, 6 cases were up-regulated in colorectal cancer tissues and 19 cases were down-regulated (76%, 19/25). Three cases were upregulated in colorectal adenoma, down-regulated in seven cases, down-regulated by 70% (7/10). Positive expression rate of MIF protein in colorectal cancer tissues was 18%, positive rate was 72%, positive rate was 50% in colorectal adenoma tissues, positive rate was 32%, the difference was statistically significant (P <0.05). The expression of miR-451 and MIF was correlated with the differentiation of colorectal cancer (P <0.05), but not with age, sex, Dukes stage, histological type, depth of invasion, lymph node metastasis. Conclusion: The low expression of miR-451 in colorectal carcinoma and colorectal adenoma tissue may inhibit the occurrence and development of colorectal cancer by inhibiting MIF negatively.