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目的建立测定保健食品中维生素B12的液相色谱-串联质谱法。方法样品添加内标人参皂苷Re溶液,固相萃取法(SPE)对试样进行富集、净化,以甲醇(A)和纯水(B)为流动相经Bio Basic-18 PIONEER柱(150 mm×2.1 mm,5μm)梯度洗脱分离,串联离子阱质谱在电喷雾电离正离子(ESI+)-全扫描(full)-二级质谱(MS/MS)模式下按内标法测定。结果维生素B12在50～500 ng/ml范围内具有良好的线性,相关系数r=0.992,回收率75.2%～89.5%,精密度3.6%～5.9%,检出限为5 ng/g,定量限为16 ng/g。结论本法可应用于保健食品的检测或产品质量控制。
Objective To establish a liquid chromatography-tandem mass spectrometry method for the determination of vitamin B12 in health foods. Methods The samples were packed with internal standard ginsenoside Re solution and solid phase extraction (SPE). The samples were concentrated and purified with a mobile phase of methanol (A) and pure water (B) through a Bio Basic-18 PIONEER column × 2.1 mm, 5 μm). The tandem ion trap mass spectra were determined by internal standard method in electrospray ionization positive ion (ESI +) - full scan-second order mass spectrometry (MS / MS) Results Vitamin B12 showed a good linearity in the range of 50-500 ng / ml with a correlation coefficient of 0.992. The recoveries ranged from 75.2% to 89.5% with a precision of 3.6% -5.9%. The limit of detection was 5 ng / g. The limit of quantification 16 ng / g. Conclusion This method can be applied to health food testing or product quality control.