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79年以来,从武夷山等地采集土样403份,用多种方法分离2232个放线菌株,从中筛选出田间防治小麦赤霉病效果达50％以上的13个菌株,其中1465菌株盆栽防治效果达87.18％、田间防效达91.30％,与1000倍的多菌灵相近。试验结果认为,采用“护颖法”初筛、“离体麦穗法”复筛、盆栽(或田间)植株三筛,再经大田小区试验,这一筛选模式既可在较短的时间内对大量菌株进行筛选,又能较好地防止具有内吸渗透性能的菌株漏筛,还能在初筛阶段观察对颖壳是否产生药害等。
In the past 79 years, 403 soil samples were collected from Wuyishan and other places. A total of 2232 actinomycetes strains were isolated by various methods. Thirteen strains were screened from the field for the control of wheat scab over 50% Up to 87.18%, field control effect of 91.30%, and 1000 times more similar to carbendazim. According to the results of the experiment, we found that the screening model can be used in a short period of time by using the screening method of “retaining Ying method”, re-screening “in vitro wheat method”, three-screen planting in pot (or field) Screening a large number of strains, but also to better prevent the permeability of the strains with a leak sieve, but also in the initial screening stage to observe whether the pyrography produce phytotoxicity.