论文部分内容阅读
目的探讨蛛网膜下腔出血(SAH)模型大鼠血浆中游离线粒体DNA(mtDNA)的水平及脑组织中Toll样受体-9/丝裂原蛋白激酶(TLR-9/MAPK)的表达及意义。方法将40只大鼠随机分为SAH组和假手术(Sham)组,每组20只。SAH组采用血管内穿刺法建立SD大鼠SAH动物模型,Sham组仅穿刺但不造成蛛网膜下腔出血。模型建立成功24h后,采用荧光定量实时PCR检测大鼠外周血中血浆游离mtDNA的表达量;采用逆转录PCR(RT-PCR)和免疫组化法分别检测大鼠脑组织中TLR-9、P38 MAPK的表达。结果 SAH组大鼠在建模24h后血浆中游离mtDNA表达量高于Sham组(P<0.05);RT-PCR及免疫组化检测均显示脑组织中TLR9、P38 MAPK表达较Sham组增多(P<0.05)。结论血浆游离mtDNA与脑组织中TLR-9/MAPK的表达存在协同升高的现象,mtDNA可能通过TLR-9/MAPK通路介导脑组织局部及全身性炎症反应的发生。
Objective To investigate the plasma level of mitochondrial DNA (mtDNA) and the expression of TLR-9 / MAPK in subarachnoid hemorrhage (SAH) model rats . Methods Forty rats were randomly divided into SAH group and sham group (n = 20). In the SAH group, the animal model of SAH was established by intravascular puncture in rats. Sham group only punctured but did not cause subarachnoid hemorrhage. After successful establishment of the model for 24h, the expression of plasma free mtDNA in peripheral blood of rats was detected by real-time fluorescent quantitative PCR. The expression of TLR-9 and P38 in brain tissue were detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry MAPK expression. Results The expression of free mtDNA in plasma of SAH group was higher than that of Sham group (P <0.05) after 24 h of modeling. The expression of TLR9 and P38 MAPK in brain tissue of rats in SAH group was higher than that in Sham group by RT-PCR and immunohistochemistry <0.05). Conclusion There is a synergistic increase in the expression of TLR-9 / MAPK in the plasma free mtDNA and brain tissue. The mtDNA may mediate the local and systemic inflammatory response through the TLR-9 / MAPK pathway.