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通过生理学和药理学方法,探讨了大豆根系吸收亚硒酸盐的生理特性。结果表明:大豆离体根吸收亚硒酸盐速率随pH升高而降低。pH3.0和8.0时,大豆吸收亚硒酸盐速率随硒含量增加呈比例升高,数据符合直线方程。pH5.0时,大豆吸收亚硒酸盐随硒含量增加而升高直至饱和,数据符合米氏动力学曲线方程。pH3.0时,DNP、NaF和4℃抑制亚硒酸盐吸收的程度分别为15%、19%和23%;pH5.0时,分别达到81%、80%和79%;pH8.0时,分别为16%、8%和11%。进一步研究表明,pH3.0时,HgCl2和AgNO3抑制亚硒酸盐吸收的程度分别为78%和79%;pH5.0时,磷饥饿显著促进亚硒酸盐吸收;pH8.0时,NPPB、9-CA、NPAA、TEACl、NA和DIDS分别抑制亚硒酸吸收的程度为13%、17%、22%、7%、18%和6%。表明大豆根系能通过水通道以被动方式吸收H2SeO3,通过磷转运蛋白以主动方式吸收HSeO-3。
Physiological and pharmacological methods were used to investigate the physiological characteristics of soybean selenite. The results showed that the rate of selenite absorption by soybean roots decreased with the increase of pH. At pH 3.0 and 8.0, the rate of selenite uptake by soybeans increased with the increase of selenium content, and the data were in accordance with the linear equation. At pH5.0, selenite uptake by soybeans increased with selenium content increasing until it saturates, and the data fit the Mie kinetic curve equation. At pH 3.0, the extent of inhibition of selenite uptake by DNP, NaF and 4 ° C was 15%, 19% and 23%, respectively; at pH 5.0 they reached 81%, 80% and 79%, respectively; at pH 8.0 , Respectively 16%, 8% and 11%. Further studies showed that, at pH3.0, HgCl2 and AgNO3 inhibited selenite uptake by 78% and 79%, respectively; at pH5.0, phosphorus starvation significantly promoted selenite uptake; at pH8.0, NPPB, The levels of selenite absorption inhibited by 9-CA, NPAA, TEAC1, NA and DIDS were 13%, 17%, 22%, 7%, 18% and 6%, respectively. Indicating that soybean roots can passively absorb H2SeO3 through the water channel and actively adsorb HSeO-3 through the phosphate transporter.