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我室曾建立分泌抗Ｂ血型抗原ＩｇＭ类单克隆抗体的5Ｄ12杂交瘤细胞株[1] ,本文应用基因工程技术 ,分离和克隆了 5Ｄ12株单抗的ＶＨ 和ＶＬ 基因 ,采用重叠延伸拼接ＰＣＲ(ＳＯＥＰＣＲ )技术[2 ] 体外成功构建 5Ｄ12单链抗体基因 ,获得较高水平的表达。1 材料和方法1 1 细胞?
In our laboratory, a 5D12 hybridoma cell line secreting anti-B-type IgM monoclonal antibody has been established [1]. In this paper, VH and VL genes of 5D12 monoclonal antibody were isolated and cloned by using gene engineering technology. SOEPCR) technology [2] 5D12 scFv gene was successfully constructed in vitro to obtain a higher level of expression. 1 Materials and Methods 1 1 cells?