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对中国李（ＰｒｕｎｕｓｓａｌｉｃｉｎａＬｉｎｄｌ．）原生质体分离和培养的有关因素进行了研究。以悬浮培养物为材料，在适宜的酶解液中酶解１２ｈ，原生质体产量和活力分别达到３×１０７个／ｇ和９５％．以改良ＭＳ为基本培养基，在培养初期加２，４－Ｄ１．０ｍｇ／Ｌ，ＢＡ０．５ｍｇ／Ｌ，培养３０ｄ后将ＢＡ调至１．０ｍｇ／Ｌ，以０．５５ｍｏｌ／Ｌ葡萄糖调节渗透压，在２×１０５个／Ｌ的植板密度下液体浅层培养５０ｄ后形成了微愈伤组织。微愈伤组织转至固体培养基上继代培养，在分化培养基上分化出不定芽，在生根培养基上生根形成完整植株。
The factors related to the isolation and culture of protoplasts from Prunus sulcalina Lindl. Were studied. The suspension cultures were used as materials and enzymatically hydrolyzed in the appropriate enzyme solution for 12 h. The protoplast yield and viability reached 3 × 107 cells / g and 95%, respectively. To improve the MS as the basic medium, in the early stages of culture plus 2,4-D1.0mg / L, BA0.5mg / L, after 30d BA adjusted to 1.0mg / L, with 0.55mol / L glucose to adjust the penetration The micro-callus was formed after shallow liquid culture for 50 days under the density of 2 × 105 / L. Micro-callus was transferred to solid medium for subculture, adventitious buds were differentiated on differentiation medium and rooted on rooting medium to form intact plants.