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建立了测定松子中黄曲霉毒素B1、B2、G1、G 2的高效液相色谱方法。方法以甲醇-水(8+2)提取,免疫亲和层析柱净化,碘液柱后衍生,荧光检测器进行检测。试验结果表明:黄曲霉毒素B1、G1标准溶液浓度在1—50Lg/L范围内,黄曲霉毒素B2、G2标准溶液浓度在0.3—15Lg/L范围内,线性良好。黄曲霉毒素B1、B2、G1、G2的检出限分别为0.10、0.05、0.10、0.10Lg/kg,平均回收率为85.00%—96.20%,精密度<5%。
A HPLC method for the determination of aflatoxins B1, B2, G1, G2 in pine nuts was established. The method was extracted with methanol - water (8 + 2), purified by immunoaffinity chromatography, derivatized with iodine solution and detected by fluorescence detector. The results showed that the concentration of aflatoxins B1 and G1 standard solution was in the range of 1-50 Lg / L, the aflatoxins B2 and G2 standard solution concentration was in the range of 0.3-15 Lg / L, and the linearity was good. The detection limits of aflatoxins B1, B2, G1 and G2 were 0.10, 0.05, 0.10 and 0.10Lg / kg, respectively. The average recoveries were 85.00% -96.20% and the precision was less than 5%.