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目的:研究视网膜母细胞瘤易感基因(Rb)在胃癌组织中的表达,探讨其与临床病理因素的关系及对预后的影响。方法:选取10年间经手术切除并经病理证实的早期和进展期胃癌73例,并对其蜡块进行免疫组化SP法染色;检测Rb在胃癌组织中的表达,分析其与临床病理指标、预后的关系。结果:⑴胃癌组织中存在Rb,阳性率为53.4%;Rb与病期、胃癌浸润深度、淋巴结转移及肿瘤分化程度有关,并与术后5年生存时间有关(P<0.05);⑵Kaplan-Meier生存分析显示:Rb阳性表达的累计生存率明显低于阴性表达;⑶单变量分析显示:Rb的表达、肿瘤大小、病期、组织学类型、淋巴结转移、浸润深度及远处转移均影响胃癌预后;多变量分析表明,只有淋巴结转移和浸润深度是显著独立的预后指标。结论:⑴Rb在胃癌的形成和发展中起协同促进作用,影响胃癌生长、分化,参与胃癌进展和转移过程;⑵Rb在一定程度上影响胃癌的预后,其过度表达提示胃癌预后差。
Objective: To investigate the expression of retinoblastoma gene (Rb) in gastric carcinoma and its relationship with clinicopathological parameters and prognosis. Methods: Seventy-three cases of early and advanced gastric cancer surgically resected and pathologically confirmed during 10 years were selected and immunohistochemically stained with SP immunohistochemistry. The expression of Rb in gastric cancer tissues was detected and compared with clinicopathological parameters, Prognosis of the relationship. Results: (1) The positive rate of Rb in gastric cancer was 53.4%. Rb was related to the disease stage, the depth of gastric cancer invasion, lymph node metastasis and tumor differentiation, and was related to the 5-year survival time (P <0.05). (2) Kaplan-Meier Survival analysis showed that the cumulative survival rate of Rb positive expression was significantly lower than that of negative expression. (3) Univariate analysis showed that the expression of Rb, tumor size, disease stage, histological type, lymph node metastasis, depth of invasion and distant metastasis all affected the prognosis Multivariate analysis showed that only lymph node metastasis and depth of invasion were significantly independent prognostic indicators. (1) Rb plays a synergistic role in promoting the formation and development of gastric cancer, affecting the growth and differentiation of gastric cancer, and participating in the progression and metastasis of gastric cancer; (2) Rb affects the prognosis of gastric cancer to a certain extent. Overexpression of Rb suggests poor prognosis of gastric cancer.