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目的:研究槟榔种子总酚类物质的抗抑郁作用并探讨其作用机制。方法:连续灌胃给药15 d,采用小鼠悬尾、强迫游泳等抑郁模型,以小鼠行为绝望的不动时间作为指标,从动物学行为上考察槟榔种子总酚类抗抑郁活性;通过HPLC-ECD法测定小鼠脑组织中单胺类递质肾上腺素(E)、去甲肾上腺素(NE)、多巴胺(DA)、五羟色胺(5-HT)含量。结果:与空白对照组比较,槟榔种子总酚类320、160 mg/kg均能显著减少小鼠悬尾和强迫游泳的不动时间,80 mg/kg无明显作用;槟榔种子总酚类320 mg/kg组与氟西汀组NE含量均显著增加,5-HT含量有所增加,DA以及E含量有所减少,但差异无统计学意义。结论:槟榔种子总酚类具有一定的抗抑郁作用。
Objective: To study the antidepressant effect of total phenolic compounds in betel nut and to explore its mechanism. Methods: The mice were subjected to continuous gavage for 15 days. The mice were subjected to the tail suspension and forced swimming, and the immobility time of mice desperation was taken as the index to study the total phenolic antidepressant activity of betel nut from zoological behavior. The contents of monoamine neurotransmitter (NE), dopamine (DA) and 5-hydroxytryptamine (5-HT) in brain were measured by HPLC-ECD. Results: Compared with the blank control group, total phenols of betel nut seed at 320 mg / kg and 160 mg / kg all reduced immobility time of mice tail suspension and forced swimming obviously, while 80 mg / kg had no obvious effect. / kg group and fluoxetine group NE content increased significantly, 5-HT content increased, DA and E content decreased, but the difference was not statistically significant. Conclusion: The total phenolics of areca nut have some antidepressant effect.